Recombinant Rabbit Anti-PRMT1 Antibody (clone R08-3H8)
CAT#: VS3-FY1200
This product is a recombinant rabbit antibody that recognizes PRMT1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin. The clone R08-3H8 is specific for human, rat PRMT1. The antigen is human prmt1 synthetic peptide.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/72136/1476_A6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69769/163033_B_7_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22550/52685_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126457-PRMT1/subcellular
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WB
Figure 1. Recombinant Rabbit Anti-PRMT1 Antibody (clone R08-3H8) in WB.
Western Blot analysis of PRMT1 in K562, C6 lysates using PRMT1 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-PRMT1 Antibody (clone R08-3H8) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PRMT1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Human PRMT1 synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Rat PRMT1
- Species Reactivity
- Human, Rat
- Clone
- R08-3H8
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- Calculated MW: 42 kDa; Observed MW: 42 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Target
- Alternative Names
- ANM 1; ANM1; ANM1_HUMAN; complete cds; HCP 1; HCP1; Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like 2; Heterogeneous nuclear ribonucleoproteins methyltransferase like2; Histone-arginine N-methyltransferase PRMT1; HMT 2; HMT1 (hnRNP methyltransferase; HMT1 (hnRNP methyltransferase S. cerevisiae) like 2; HMT1 hnRNP methyltransferase; HMT1 hnRNP methyltransferase like 2 (S. cerevisiae); HMT1 hnRNP methyltransferase like 2; HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae); HMT2; HRMT1 L2; HRMT1L 2; HRMT1L2; Human mRNA for suppressor for yeast mutant; Human mRNA for suppressor for yeast mutant complete cds; Interferon receptor 1 bound protein 4; Interferon receptor 1 bound protein4; Interferon receptor 1-bound protein 4; Interferon receptor 1bound protein 4; IR1 B4; IR1B 4; IR1B4; Mrmt 1; Mrmt1; PRMT 1; PRMT1; Protein arginine methyltransferase 1; Protein arginine N methyltransferase 1; Protein arginine N methyltransferase1; Protein arginine N-methyltransferase 1; R1B4; S. cerevisiae like 2
- Gene ID
- 3276
- UniProt ID
- Q99873
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PRMT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-802-F(E) | Recombinant Anti-human PRMT1 Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-802-F(E) | Recombinant Human Anti-human PRMT1 Antibody Fab Fragment | ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-802-S(P) | Recombinant Anti-human PRMT1 Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| MHH-802-S(P) | Recombinant Human Anti-human PRMT1 Antibody scFv Fragment | WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-802 | Recombinant Human Anti-human PRMT1 Antibody | ELISA, IP, FuncS | IgG |
| MOB-1690CT | Recombinant Mouse anti-Human PRMT1 Monoclonal antibody (NBU-C23) | ChIP, WB | |
| MRO-1267-CN | Recombinant Rabbit Anti-PRMT1 Monoclonal Antibody (JA11-17) | WB, IF, IHC, ChIP | Rabbit IgG |
| ZG-0399C | Mouse Anti-PRMT1 Recombinant Antibody (ZG-0399C) | WB | Mouse IgG |
| ZG-0400C | Mouse Anti-PRMT1 Recombinant Antibody (ZG-0400C) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2868 | Hi-Affi™ Recombinant Rabbit Anti-PRMT1 Monoclonal Antibody (DS2868AB) | IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1138 | AbPlus™ Anti-PRMT1 Magnetic Beads (VS-0724-YC1138) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY408 | Mouse Anti-NHP PRMT1 Recombinant Antibody (clone PRMT1-171) | WB, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1760 | Anti-PRMT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5719 | Anti-Mouse PRMT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5718 | Anti-Human PRMT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5720 | Anti-Rat PRMT1 Immunohistochemistry Kit | IHC |
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