Recombinant Rabbit Anti-PTK2B Antibody (clone R03-8D2) (CAT#: VS3-FY1222)
This product is a recombinant rabbit antibody that recognizes PTK2B. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R03-8D2 is specific for human PTK2B. The antigen is synthetic peptide of human pyk2.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3850/624_D4_2_selected.jpg

(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/ihc_selected.jpg

(Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_B_7_5.jpg

(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_3.jpg

(Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Hepatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_4.jpg

(Cells in glomeruli
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_5.jpg

(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_4_6.jpg

(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Squamous epithelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_6_8.jpg

(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_9_8.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000120899-PTK2B/subcellular
Specifications
- Immunogen
- Synthetic peptide of human PYK2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PTK2B
- Species Reactivity
- Human
- Clone
- R03-8D2
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 116 kDa; Observed MW: 116 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- PTK2B; FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Pro
- Gene ID
- 2185
- UniProt ID
- Q14289
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PTK2B"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1616z | Mouse Anti-PTK2B Recombinant Antibody (clone 27C3) | WB, ELISA, IHC | Mouse IgG2a |
MOB-1733CT | Recombinant Mouse anti-Human PTK2B Monoclonal antibody (EML069) | WB | |
VS7-0425-WR713 | Mouse Anti-PTK2B Monoclonal Antibody (VS7-0425-WR713) | WB, IHC-P, IF, ELISA | Mouse IgG |
VS7-0425-WR714 | Mouse Anti-PTK2B Recombinant Antibody (VS7-0425-WR714) | WB | Mouse IgG |
VS7-0425-WR730 | Mouse Anti-PTK2B Monoclonal Antibody (clone 5E2D5) | WB, IHC | Mouse IgG2a |
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