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  • Recombinant Rabbit Anti-PTK2B Antibody (clone R03-8D2)

Recombinant Rabbit Anti-PTK2B Antibody (clone R03-8D2) (CAT#: VS3-FY1222)

This product is a recombinant rabbit antibody that recognizes PTK2B. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R03-8D2 is specific for human PTK2B. The antigen is synthetic peptide of human pyk2.


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Specific Inquiry
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  • Conjugation:
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  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3850/624_D4_2_selected.jpg
Figure 2 IHC staining of human cerebral cortex
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_B_7_5.jpg
Figure 4 Colon
Figure 4 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_3.jpg
Figure 5 Liver
Figure 5 Liver
(Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Hepatocytes
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_4.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in glomeruli
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_8_5.jpg
Figure 7 Testis
Figure 7 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_4_6.jpg
Figure 8 Tonsil
Figure 8 Tonsil
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Squamous epithelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_6_8.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26091/58711_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (Daudi, REH)
Figure 10 RNA cell line category: Cell line enhanced (Daudi, REH)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000120899-PTK2B/subcellular

Specifications

  • Immunogen
  • Synthetic peptide of human PYK2.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human PTK2B
  • Species Reactivity
  • Human
  • Clone
  • R03-8D2
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 116 kDa; Observed MW: 116 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • PTK2B; FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Pro

Recommended Products

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "PTK2B"

Recombinant Antibody

CAT Product Name Application Type
MOB-1616z Mouse Anti-PTK2B Recombinant Antibody (clone 27C3) WB, ELISA, IHC Mouse IgG2a
MOB-1733CT Recombinant Mouse anti-Human PTK2B Monoclonal antibody (EML069) WB
MRO-1301-CN Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (CBACN-469) WB, IF, IHC Rabbit IgG
HPAB-1397-FY Mouse Anti-PTK2B Recombinant Antibody (HPAB-1397-FY) WB, Inhib, FC Mouse IgG
VS3-WK955 Rabbit Anti-PTK2B Recombinant Antibody (VS3-WK955) WB, ICC, IF, IHC Rabbit IgG

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0754MZ Chicken Anti-FAK2 Polyclonal IgY ELISA, IHC, IP, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2925 Hi-Affi™ Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (DS2925AB) WB, IP IgG
MOR-4574 Hi-Affi™ Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (TH86DS) WB, IF, ICC, IHC-P, FC, ChIP IgG
MOR-4681 Hi-Affi™ Recombinant Rabbit Anti-PTK2B Monoclonal Antibody (TH195DS) WB, ChIP IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-1397-FY-F(E) Mouse Anti-PTK2B Recombinant Antibody; Fab Fragment (HPAB-1397-FY-F(E)) WB, FC Mouse Fab

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-1397-FY-S(P) Mouse Anti-PTK2B Recombinant Antibody; scFv Fragment (HPAB-1397-FY-S(P)) WB, FC Mouse scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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