Recombinant Rabbit Anti-RAB7A Antibody (clone R07-8G1)
CAT#: VS3-FY1236
This product is a recombinant rabbit antibody that recognizes RAB7A. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-8G1 is specific for human, mouse, rat RAB7A. The antigen is synthetic peptide of human rab7.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to lysosomes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6964/22_B1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37131/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37131/80565_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37131/80565_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37131/80565_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37131/80565_A_6_6.jpg
Normal Tissue
Figure 7 Skeletal muscle
Myocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/37131/80565_B_3_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000075785-RAB7A
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IHC-P
Figure 1. Recombinant Rabbit Anti-RAB7A Antibody (clone R07-8G1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded mouse colon using Rab7 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
WB
Figure 2. Recombinant Rabbit Anti-RAB7A Antibody (clone R07-8G1) in WB.
Western Blot analysis of RAB7 in K562, C6, 3T3, Hela lysates using RAB7 Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human RAB7.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat RAB7A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-8G1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 23 kDa; Observed MW: 23 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- RAB7A, Member RAS Oncogene Family; RAB7, Member RAS Oncogene Family; RAB7; Ras-Associated Protein RAB7; Ras-Related Protein Rab-7a; PRO2706
- Gene ID
- 7879
- UniProt ID
- P51149
- Sequence Similarities
- Belongs to the small GTPase superfamily. Rab family.
- Cellular Localization
- Cytoplasmic vesicle, Endosome, Lipid droplet, Lysosome, Membrane, Mitochondrion
- Protein Refseq
- NP_004628.4
- Function
- Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211).
Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity).
Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Required for vesicular trafficking and cell surface expression of ACE2 (PubMed:33147445).
May play a role in PRPH neuronal intermediate filament assembly (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2479CT | Recombinant Mouse anti-Chicken RAB7A Monoclonal antibody (HD) | ICC, IF, IP, ICC, WB, ELISA | |
| MRO-1305-CN | Recombinant Rabbit Anti-RAB7A Monoclonal Antibody (CBACN-472) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-2150-CN | Rabbit Anti-RAB7A Polyclonal Antibody (MRO-2150-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ1013 | Rabbit Anti-RAB7A Recombinant Antibody (VS3-CJ1013) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| VS3-FY1235 | Recombinant Rabbit Anti-RAB7A Antibody (clone R01-3K3) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MOR-2967 | Hi-Affi™ Recombinant Rabbit Anti-RAB7A Monoclonal Antibody (DS2967AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
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| VS-0724-YC246 | AbPlus™ Anti-RAB7A Magnetic Beads (VS-0724-YC246) | IP, Protein Purification | |
| VS-0724-YC341 | AbPlus™ Anti-Rab7a Magnetic Beads (VS-0724-YC341) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS13-YC971 | CytoStream™ Rabbit Anti-RAB7A Recombinant Antibody (VS13-YC971) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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