Recombinant Rabbit Anti-SOX2 Capture Antibody (clone R85-4Y-7) (CAT#: VS3-WK1928)
This antibody is a rabbit recombinant antibody against human SOX2. It was developed by immunization of rabbit with recombinant human SOX2 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R85-4Y-7 and R92-8O-9. The clone R85-4Y-7 can be used as a capture antibody in ELISA analysis to identify human SOX2.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45725/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45725/if_selected.jpg
Figure 2 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62851/1273_B4_7_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62851/1273_B4_7_selected.jpg
Figure 3 High expression in cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_9_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_9_5.jpg
Figure 4 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_6_1.jpg
Figure 5 Colon
(Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_A_7_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (AF22, HAP1, NTERA-2, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181449-SOX2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181449-SOX2
Specifications
- Immunogen
- Recombinant human SOX2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SOX2
- Species Reactivity
- Human
- Clone
- R85-4Y-7
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- SRY-Box 2; SRY (Sex Determining Region Y)-Box 2; Sex Determining Region Y-Box 2; SRY-Related HMG-Box Gene 2; Transcription Factor SOX-2; Transcription Factor SOX2; SRY Box 2; MCOPS3; ANOP3
- Gene ID
- 6657
- UniProt ID
- P48431
- Long Name
- SRY-Box 2
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation.
- Protein Refseq
- NP_003097.1
- Function
- Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206.
Binds to the proximal enhancer region of NANOG.
Recommended Products
Detection antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2438z | Mouse Anti-SOX2 Recombinant Antibody (clone 44A5) | WB, ELISA, IHC | Mouse IgG2b, κ |
| MOB-1906CT | Recombinant Mouse anti-Human SOX2 Monoclonal antibody (68DU34.4.5) | ELISA, ICC/IF, IHC-P, WB, sELISA | |
| VS3-QX1042 | Mouse Anti-SOX2 Recombinant Antibody (clone 2A10-F11-F12) | WB, FC, ICC | Mouse IgG1 |
| VS3-QX1043 | Mouse Anti-SOX2 Recombinant Antibody (clone 57CT23.3.4) | WB, ICC, IHC, FC | Mouse IgG1 |
| VS3-QX1044 | Mouse Anti-SOX2 Recombinant Antibody (clone 10F10C9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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