Recombinant Rabbit Anti-TARDBP Detection Antibody (clone R95-6N-1) (CAT#: VS3-WK1939)
This antibody is a rabbit recombinant antibody against human TARDBP. It was developed by immunization of rabbit with recombinant human TARDBP protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R98-7T-3 and R95-6N-1 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R95-6N-1 can be used in ELISA analysis to identify human TARDBP.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/138_C12_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/138_C12_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/662_G6_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/662_G6_1_selected.jpg
Figure 3 IHC staining of human epididymis
(Immunohistochemical staining of human epididymis shows distinct positivity in both nuclei and cytoplasm of glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/ihc_selected.jpg
(Immunohistochemical staining of human epididymis shows distinct positivity in both nuclei and cytoplasm of glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/ihc_selected.jpg
Figure 4 IHC staining of human pancreas
(Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/ihc_selected.jpg
(Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/ihc_selected.jpg
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/166_C12_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/166_C12_1_red_green.jpg
Figure 6 Cerebral cortex
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_B_8_5.jpg
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_B_8_5.jpg
Figure 7 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_3.jpg
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_3.jpg
Figure 8 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_4.jpg
Figure 9 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_5.jpg
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_5.jpg
Figure 10 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_6_6.jpg
(Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_6_6.jpg
Figure 11 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_8.jpg
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120948-TARDBP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120948-TARDBP
Specifications
- Immunogen
- Recombinant human TARDBP protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TARDBP
- Species Reactivity
- Human
- Clone
- R95-6N-1
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- TAR DNA Binding Protein; TDP-43; TAR DNA-Binding Protein 43; TAR DNA-Binding Protein-43; ALS10; TDP43
- Gene ID
- 23435
- UniProt ID
- Q13148
- Long Name
- TAR DNA Binding Protein
- Cellular Localization
- Cytoplasm, Mitochondrion
, Nucleus
- Post Translation Modifications
- Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Phosphorylated upon cellular stress.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
- Protein Refseq
- NP_031401.1
- Function
- RNA-binding protein that is involved in various steps of RNA biogenesis and processing.
Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-2370-VHH | Recombinant Anti-Human TARDBP VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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