Recombinant Rabbit Anti-TBP Antibody (clone R04-6D1)
CAT#: VS3-FY1430
This product is a recombinant rabbit antibody that recognizes TBP. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R04-6D1 is specific for human, mouse TBP. The antigen is synthetic peptide of human tata-binding protein tbp.
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-TBP Antibody (clone R04-6D1) in WB.
Western Blot analysis of TATA-binding protein TBP in 3T3 lysates using TATA Box Binding Protein Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-TBP Antibody (clone R04-6D1) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human tonsil using antibody to TATA-binding protein TBP. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49805/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9442/if_selected.jpg
Normal Tissue
Figure 3 Urinary bladder
Urothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49805/146472_A_5_5.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112592-TBP
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Specifications
- Immunogen
- Synthetic peptide of human TATA-binding protein TBP.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse TBP
- Species Reactivity
- Human, Mouse
- Clone
- R04-6D1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 38 kDa; Observed MW: 38 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- TATA-Box Binding Protein; Transcription Initiation Factor TFIID TBP Subunit; TATA Sequence-Binding Protein; TATA-Box Factor; GTF2D1; TFIID; TATA-Box Binding Protein N-Terminal Domain
- Gene ID
- 6908
- UniProt ID
- P20226
- Sequence Similarities
- Belongs to the TBP family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_001165556.1; NP_003185.1
- Function
- The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473).
TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) (PubMed:33795473, PubMed:27193682, PubMed:2194289, PubMed:2363050, PubMed:2374612).
The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473, PubMed:27007846).
The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C (PubMed:33795473).
TBP forms the TFIID-A module together with TAF3 and TAF5 (PubMed:33795473).
TBP is a general transcription factor that functions at the core of the TFIID complex (PubMed:33795473, PubMed:27193682, PubMed:2194289, PubMed:2363050, PubMed:2374612, PubMed:9836642).
During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box (PubMed:33795473).
Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071).
Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription (PubMed:15970593).
The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter (PubMed:15970593).
SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA (PubMed:15970593).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
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- Product Name
Isotype Control
- CAT
- Product Name
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