Recombinant Rabbit Anti-TPX2 Antibody (clone R01-8A6)
CAT#: VS3-FY1482
This product is a recombinant rabbit antibody that recognizes TPX2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The antigen is synthetic peptide of human TPX2. The clone R01-8A6 is specific for human TPX2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, cytokinetic bridge & mitotic spindle.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5487/1825_A5_31_cr5ac33e6e0739c_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5487/15405_B_8_5.jpg
Normal Tissue
Figure 3 Lymph node
nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5487/15405_A_9_8.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000088325-TPX2
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Specifications
- Immunogen
- Synthetic peptide of human TPX2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPX2
- Species Reactivity
- Human
- Clone
- R01-8A6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 86 kDa; Observed MW: 100 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- TPX2, Microtubule Nucleation Factor; Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2; Hepatocellular Carcinoma-Associated Antigen 519; Hepatocellular Carcinoma-Associated Antigen 90; Protein Fls353; C20orf1; C20orf2; HCA519; DIL-2; P100; DIL2; Restricted Expression Proliferation Associated Protein 100; Restricted Expression Proliferation-Associated Protein 100
- Gene ID
- 22974
- UniProt ID
- Q9ULW0
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "TPX2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1819MZ | Recombinant Mouse Anti-Human TPX2 Antibody (clone 29E6) | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1163 | Mouse Anti-TPX2 Recombinant Antibody (clone 3H10-F8-D7) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1164 | Mouse Anti-TPX2 Recombinant Antibody (clone 19AT738.292) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1211 | Mouse Anti-TPX2 Recombinant Antibody (VS3-WK1211) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1212 | Mouse Anti-TPX2 Recombinant Antibody (VS3-WK1212) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2342 | Anti-TPX2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7483 | Anti-Mouse TPX2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7482 | Anti-Human TPX2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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