Recombinant Rabbit Anti-YBX1 Antibody (clone R03-6G4) (CAT#: VS3-FY1568)

• Immunogen
• Human YB1 synthetic peptide.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Hamster YBX1

• Species Reactivity
• Human, Mouse, Hamster

• Clone
• R03-6G4

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 36 kDa; Observed MW: 49 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• Y-Box Binding Protein 1; CCAAT-Binding Transcription Factor I Subunit A; Enhancer Factor I Subunit A; Y-Box Transcription Factor; DNA-Binding Protein B; CBF-A; NSEP1; EFI-A; DBPB; YB-1; YB1

• Gene ID
• 4904

• UniProt ID
• P67809

• Sequence Similarities
• Belongs to the YBX1 family.

• Cellular Localization
• Cytoplasm, Nucleus, Secreted

• Post Translation Modifications
• Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcactivational ability.
  In the absence of phosphorylation the protein is retained in the cytoplasm.
  Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus (By similarity).

• Protein Refseq
• NP_004550.2

• Function
• DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed:8188694, PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969).
  Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:19561594, PubMed:31358969).
  Promotes mRNA stabilization: acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed:10817758, PubMed:11698476, PubMed:31358969).
  Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed:19029303).
  Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity).
  Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed:27559612, PubMed:29073095).
  Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs (PubMed:28341602, PubMed:29073095).
  Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed:29712925).
  Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection (PubMed:12604611).
  Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7' (PubMed:18809583).
  Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed:8188694, PubMed:18809583).
  Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed:14718551).
  Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair (PubMed:14718551).
  The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed:19483673).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody