CytoStream™ Rabbit Anti-YBX1 Recombinant Antibody (VS13-YC1233) (CAT#: VS13-YC1233)

• Immunogen
• Recombinant human YB1, aa 275-324.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Mouse YBX1

• Species Reactivity
• Human, Mouse

• Applications
• WB, ICC, IF, IHC-P, FC, IP

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/1000-1/5000
  Immunocytochemistry (ICC): 1/100-1/500
  Immunofluorescence (IF): 1/100-1/500
  Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
  Flow cytometry (FC): 1/50-1/100

• Alternative Names
• Y-Box Binding Protein 1; CCAAT-Binding Transcription Factor I Subunit A; Enhancer Factor I Subunit A; Y-Box Transcription Factor; DNA-Binding Protein B; CBF-A; NSEP1; EFI-A; DBPB; YB-1; YB1;

• Gene ID
• 4904

• UniProt ID
• P67809

• Long Name
• Y-Box Binding Protein 1

• Sequence Similarities
• Belongs to the YBX1 family.

• Cellular Localization
• Secreted; Nucleus; Cytoplasm; Cytoplasmic granule

• Protein Refseq
• NP_004550.2

• Function
• DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed:8188694, PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969).
  Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:19561594, PubMed:31358969).
  Promotes mRNA stabilization: acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed:10817758, PubMed:11698476, PubMed:31358969).
  Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed:19029303).
  Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity).
  Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed:27559612, PubMed:29073095).
  Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs (PubMed:28341602, PubMed:29073095).
  Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed:29712925).
  Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection (PubMed:12604611).
  Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7' (PubMed:18809583).
  Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed:8188694, PubMed:18809583).
  Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed:14718551).
  Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair (PubMed:14718551).
  The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed:19483673).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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