Hi-Affi™ Rabbit Anti-ADGRE5 Recombinant Antibody (clone DS594AB)
CAT#: MOR-0594
Recombinant Rabbit anti-CD97 monoclonal antibody for WB, IHC, ICC, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/if_selected.jpg
Normal Tissue
Figure 2 High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_4_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_6_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_4_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123146-ADGRE5
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Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS594AB
- Applications
- WB, IHC, ICC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Adhesion G Protein-Coupled Receptor E5; Leukocyte Antigen CD97; Seven Transmembrane Helix Receptor; Seven-Span Transmembrane Protein; CD97 Molecule; CD97 Antigen; Seven-Transmembrane, Heterodimeric Receptor Associated With Inflammation; Heterodimeric Receptor Associated With Inflammation; Seven-Transmembrane; TM7LN1; CD97
- Gene ID
- 976
- UniProt ID
- P48960
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ADGRE5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2433z | Mouse Anti-ADGRE5 Recombinant Antibody (clone 31A9) | WB, ELISA | Mouse IgG2b, κ |
| ZG-0600J | Rabbit Anti-ADGRE5 Recombinant Antibody (clone 3A7) | ELISA, IHC, IF | Rabbit IgG |
| VS3-XY3253 | Rabbit Anti-ADGRE5 Recombinant Antibody (clone R05-4Z1) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-010CQ | Mouse Anti-ADGRE5 Recombinant Antibody (NEUT-010CQ) | Neut | Mouse IgG2 |
| NEUT-011CQ | Mouse Anti-ADGRE5 Recombinant Antibody (clone CBL269) | Neut, WB, FC, ELISA | Mouse IgG |
| NEUT-012CQ | Mouse Anti-ADGRE5 Recombinant Antibody (clone CBL373) | Neut, FC, CyTOF, WB | Mouse IgG2a |
| NEUT-013CQ | Mouse Anti-ADGRE5 Recombinant Antibody (clone CBL767) | ELISA(Cap), Neut | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-014CQ | Rat Anti-ADGRE5 Recombinant Antibody (clone CBL379) | Block, CyTOF, FC | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC372 | AbPlus™ Anti-Adgre5 Magnetic Beads (VS-0724-YC372) | IP, Protein Purification | |
| VS-0724-YC456 | AbPlus™ Anti-ADGRE5 Magnetic Beads (VS-0724-YC456) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY170 | Anti-ADGRE5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY171 | Anti-Rat ADGRE5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY172 | Anti-Mouse ADGRE5 Immunohistochemistry Kit | IHC |
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