Rabbit Anti-ADGRE5 Recombinant Antibody (clone R05-4Z1) (CAT#: VS3-XY3253)
The rabbit recombinant antibody specifically binds to Adhesion G protein-coupled receptor E5 (ADGRE5), which plays an essential role in leukocyte migration. It also has cross-reactivity against mouse ADGRE5. It has been detected using Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/if_selected.jpg
Figure 2 High expression in spleen
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_4_val_selected.jpg
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_4_val_selected.jpg
Figure 3 Cerebral cortex
(Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_5.jpg
(Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_9_3.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_6_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_6_6.jpg
Figure 6 Bone marrow
(Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_4_4.jpg
(Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_4_4.jpg
Figure 7 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_7_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123146-ADGRE5
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123146-ADGRE5
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse ADGRE5
- Species Reactivity
- Human, Mouse
- Clone
- R05-4Z1
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Vibratory Urticaria; Thyroid Cancer
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- CD97; TM7LN1
- Gene ID
- 976
- UniProt ID
- P48960
- Long Name
- Adhesion G protein-coupled receptor E5
- Cellular Localization
- Cell membrane
- Post Translation Modifications
- Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
- Protein Refseq
- NP_510966.1; NP_001775.2; NP_001020331.1
- Function
- Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2433z | Mouse Anti-ADGRE5 Recombinant Antibody (clone 31A9) | WB, ELISA | Mouse IgG2b, κ |
| ZG-0600J | Rabbit Anti-ADGRE5 Recombinant Antibody (clone 3A7) | ELISA, IHC, IF | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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