Rabbit Anti-ADGRE5 Recombinant Antibody (clone 3A7)
CAT#: ZG-0600J
This product is a rabbit antibody that recognizes ADGRE5.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-ADGRE5 Antibody (ZG-0600J) in IHC
IHC image of ZG-0600J diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 2 Rabbit Anti-ADGRE5 Antibody (ZG-0600J) in IHC
IHC image of ZG-0600J diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-ADGRE5 Antibody (ZG-0600J) in IF
Immunofluorescence staining of Hela cells with ZG-0600J at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/if_selected.jpg
Normal Tissue
Figure 2 High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_4_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Enterocytes Staining: Low Intensity: Moderate Quantity: <25% Enterocytes - Goblet cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_6_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30814_B_4_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13707/30524_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123146-ADGRE5
❮
❯
Specifications
- Immunogen
- A synthesized peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ADGRE5
- Clone
- 3A7
- Applications
- ELISA, IHC, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.
IHC:1:50-1:500, IF:1:30-1:200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0600J. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ADGRE5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2433z | Mouse Anti-ADGRE5 Recombinant Antibody (clone 31A9) | WB, ELISA | Mouse IgG2b, κ |
| VS3-XY3253 | Rabbit Anti-ADGRE5 Recombinant Antibody (clone R05-4Z1) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-010CQ | Mouse Anti-ADGRE5 Recombinant Antibody (NEUT-010CQ) | Neut | Mouse IgG2 |
| NEUT-011CQ | Mouse Anti-ADGRE5 Recombinant Antibody (clone CBL269) | Neut, WB, FC, ELISA | Mouse IgG |
| NEUT-012CQ | Mouse Anti-ADGRE5 Recombinant Antibody (clone CBL373) | Neut, FC, CyTOF, WB | Mouse IgG2a |
| NEUT-013CQ | Mouse Anti-ADGRE5 Recombinant Antibody (clone CBL767) | ELISA(Cap), Neut | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-014CQ | Rat Anti-ADGRE5 Recombinant Antibody (clone CBL379) | Block, CyTOF, FC | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0594 | Hi-Affi™ Rabbit Anti-ADGRE5 Recombinant Antibody (clone DS594AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC372 | AbPlus™ Anti-Adgre5 Magnetic Beads (VS-0724-YC372) | IP, Protein Purification | |
| VS-0724-YC456 | AbPlus™ Anti-ADGRE5 Magnetic Beads (VS-0724-YC456) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY170 | Anti-ADGRE5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY171 | Anti-Rat ADGRE5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY172 | Anti-Mouse ADGRE5 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
![Figure 2 Anti-Human CD19 Recombinant Antibody Fab Fragment [TAB-1611CL-F(E)] in HPLC](https://img.creativebiolabs.net/productimages/COA-TAB-1611CL-F(E)-2.png)
Application: Depletion, FuncS
Application: WB, ELISA, IF
Application: FC, FRET, Internalization
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: Neut
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, FuncS, Neut, IF, WB, EM, Inhib, IHC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
