Which buffers are suggested for the dilution of antibodies?

To stabilize the antibody, dilution can be carried out in PBS or TBS, which is frequently supplemented with 0.1% Tween-20 and occasionally with 1-5% BSA.

Is it possible to use this antibody in multiplex tests?

If it works well with the other antibodies and chemicals used in the experiment, then the answer is definitely yes for using it in multiplex assays.

What is this antibody's level of purity?

SDS-PAGE analysis usually shows that the purity is greater than 95%. Minimal contamination and trustworthy experimental results are guaranteed by high purity.

Recombinant Anti-human ALB VHH Single Domain Antibody

CAT#: NAB-81-sdAb

Anti-human ALB VHH Single Domain Antibody is a recombinant protein produced in E. coli.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line Hep G2

Immunofluorescent staining of human cell line Hep G2 shows localization to endoplasmic reticulum & the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/763_G10_1_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human fallopian tube

Immunohistochemical staining of human fallopian tube shows distinct positivity in plasma.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6262/17728_A_9_7_selected.jpg

Normal Tissue Figure 3 Cerebral cortex

Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_B_7_5.jpg

Normal Tissue Figure 4 Colon

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_3.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining:Medium
Intensity: Moderate

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_7_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31024/128566_A_6_6.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enriched (Hep G2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163631-ALB

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Specifications

Immunogen
human albumin

Host Species
Llama

Type
Llama VHH

Species Reactivity
Human

Molecule Class
VHH Single Domain Antibody

Applications
This product is suitable for use in IHC, ICC, IP, ChiP, Neut and most other immunological methods.

Product Property

Storage
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.

Target

Alternative Names
ALB; albumin; FDAH; ANALBA; PRO0883; PRO0903; PRO1341

Gene ID
213

UniProt ID
P02768

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Customer Review

There are currently no Customer reviews or questions for NAB-81-sdAb. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Strong Immunohistochemistry

We achieved excellent staining in immunohistochemistry. The antibody produced sharp, clear images. Definitely recommending to colleagues.

Effective for Immunoprecipitation

We used this antibody for immunoprecipitation, and the results were excellent. Very effective and reliable.

Ideal for Extended Projects

The datasheet that was supplied included comprehensive and easy-to-use protocols. The comprehensive guidelines were straightforward to understand and follow, even for someone who is not very experienced in antibody research.

Q&As

Which buffers are suggested for the dilution of antibodies?

A: To stabilize the antibody, dilution can be carried out in PBS or TBS, which is frequently supplemented with 0.1% Tween-20 and occasionally with 1-5% BSA.
Is it possible to use this antibody in multiplex tests?

A: If it works well with the other antibodies and chemicals used in the experiment, then the answer is definitely yes for using it in multiplex assays.
What is this antibody's level of purity?

A: SDS-PAGE analysis usually shows that the purity is greater than 95%. Minimal contamination and trustworthy experimental results are guaranteed by high purity.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NAB-81-sdAb.pdf

MSDS

MSDS - NAB-81-sdAb.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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