This single chain antibody fragment that specifically binds to CD14 with good positive cells without recognition of other organizations can be used in antiendotoxin therapy.
Figure 1 Expression of scFv2F9 in CHO cells.
(A) SDS-PAGE analysis of the scFv2F9. Lane M: Prestained Protein Molecular Weight Marker, Lane 1: supernatant of the CHO cells transfected with the pSectag2A, Lane 2: Supernatant of the CHO cells transfected with the pSectag2A/ScFv2F9. (B) Western-blot analysis of ScFv2F9. The lane symbols are the same as denoted in (A).
Tang, Y. M., Ning, B. T., Cao, J., Shen, H. Q., & Qian, B. Q. (2007). Construction and expression of single-chain antibody derived from a new clone of monoclonal antibody against human CD14 in CHO cells. Immunopharmacology and immunotoxicology, 29(3-4), 375-386.
Figure 2 The blocking test of ScFv2F9 secreted from CHO cells against 2F9-FITC.
5 groups including background control (using antigen-unrelated murine IgG1 antibody), negative control (using 2F9-FITC), positive control (purified parental 2F9 McAb), culture supernatant from pSectag2A/scFv2F9 and culture supernatant from pSectag2A were designed. 5 ×10⁵ PBMC were added to each tube suspended with 50 μl of PBS. After washing and re-suspending with 500 μl of PBS, fluorescent signals of FITC were detected through FCM. Three parameters as percentage of positive cells (Percentage), mean fluorescence intensity (MFI) and peak channel (PeakCh) were used to evaluate the blocking efficacy.
Tang, Y. M., Ning, B. T., Cao, J., Shen, H. Q., & Qian, B. Q. (2007). Construction and expression of single-chain antibody derived from a new clone of monoclonal antibody against human CD14 in CHO cells. Immunopharmacology and immunotoxicology, 29(3-4), 375-386.
Figure 3 The blocking effect of ScFv2F9 secreted from CHO cells against 2F9-FITC.
Histogram of the blocking effect of ScFv2F9 secreted from CHO cells against 2F9-FITC was shown according to the results of FCM, the ordinate presented blocking percentage, 3 parameters' percentage, peakCh and MFI were used to evaluate the blocking effect of the supernatant from CHO cell transfected with pSectag2A/ScFv2F9, 2F9 McAb and supernatant from CHO cell transfected with pSectag2A.
Tang, Y. M., Ning, B. T., Cao, J., Shen, H. Q., & Qian, B. Q. (2007). Construction and expression of single-chain antibody derived from a new clone of monoclonal antibody against human CD14 in CHO cells. Immunopharmacology and immunotoxicology, 29(3-4), 375-386.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1182z | Mouse Anti-CD14 Recombinant Antibody (clone 13B10) | ICC, IF, FuncS | Mouse IgG1 |
MRO-0279-CN | Rabbit Anti-CD14 Recombinant Antibody (clone CBACN-108) | WB, IF, IHC | Rabbit IgG |
HPAB-N0031-YC | Human Anti-CD14 Recombinant Antibody (clone P1A8) | IF, WB | Human IgG |
ZG-0536F | Mouse Anti-CD14 Recombinant Antibody (clone MEM18) | FC, IF | Mouse IgG1 |
VS3-WK1444 | Mouse Anti-CD14 Recombinant Antibody (clone W4) | FC, IF | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1588CL | Anti-Human CD14 Recombinant Antibody (Hm2F9) | FC | Chimeric antibody (mouse/human) |
TAB-1585CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (rl8D11) | FC, ELISA | Chimeric antibody (mouse/human) |
TAB-1586CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (rMil2) | FC, ELISA | Chimeric antibody (mouse/human) |
TAB-1588CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (Hm2F9) | FC | Chimeric antibody (mouse/human) |
TAB-1590CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (F1024) | FuncS | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1589CL-S(P) | Anti-Human CD14 Recombinant Antibody scFv Fragment (scFv2F9) | WB, FC, Block | Human antibody |
TAB-1589CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (scFv2F9) | WB, FC, Block | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0099MZ | Chicken Anti-CD14 Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-275CQ | Mouse Anti-CD14 Recombinant Antibody (clone CBL012) | WB, Neut | Mouse IgG1 |
NEUT-276CQ | Mouse Anti-CD14 Recombinant Antibody (clone CBL795) | Neut | Mouse IgG1 |
NEUT-277CQ | Human Anti-CD14 Recombinant Antibody (clone D3B8) | Neut, FC | Human IgA2 |
NEUT-279CQ | Mouse Anti-CD14 Recombinant Antibody (clone 4H53) | WB, Neut | Mouse IgG1 |
NEUT-280CQ | Mouse Anti-CD14 Recombinant Antibody (clone 61D3) | FC, IHC-Fr, IP, Neut | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-278CQ | Mouse Anti-CD14 Recombinant Antibody (clone UCHM-1) | FC, IHC, ICC, IP, ELISA, Block, Depletion | Mouse IgG2a, κ |
NEUT-282CQ | Mouse Anti-CD14 Recombinant Antibody (clone BiG10) | FC, ELISA, Inhib, WB | Mouse IgG |
NEUT-284CQ | Rat Anti-CD14 Recombinant Antibody (clone M14-23) | FC, Block | Rat IgG2a, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-0532 | Hi-Affi™ Rabbit Anti-CD14 Recombinant Antibody (clone DS532AB) | ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0101-YC-S(P) | Mouse Anti-CD14 Recombinant Antibody (clone 28C5); scFv Fragment | ELISA, FC, Block | Mouse scFv |
HPAB-N0031-YC-S(P) | Human Anti-CD14 Recombinant Antibody (clone P1A8); scFv Fragment | IF, WB | Human scFv |
HPAB-1538WJ-S(P) | Mouse Anti-CD14 Recombinant Antibody; scFv Fragment (clone 18E12) | ELISA, WB, FC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0101-YC-F(E) | Mouse Anti-CD14 Recombinant Antibody (clone 28C5); Fab Fragment | ELISA, FC, Block | Mouse Fab |
HPAB-N0031-YC-F(E) | Human Anti-CD14 Recombinant Antibody (clone P1A8); Fab Fragment | IF, WB | Human Fab |
HPAB-1538WJ-F(E) | Mouse Anti-CD14 Recombinant Antibody; Fab Fragment (clone 18E12) | ELISA, WB, FC | Mouse Fab |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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