Human Anti-CD14 (clone 28C5) scFv-Fc Chimera (CAT#: VS-0325-FY177)
An "anti-CD14 scFv-Fc antibody" is a recombinant antibody specifically designed to target the CD14 protein on the surface of cells, composed of a single-chain variable fragment (scFv) that provides antigen-binding specificity, fused to the Fc region of an immunoglobulin which allows for effector functions like immune cell activation and enhanced stability in the body; essentially, it is a highly targeted antibody that can bind to CD14-expressing cells and trigger immune responses against them, primarily used in research to study immune cell function and potential therapeutic applications related to inflammation and infection.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/1183_E12_3_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/1183_E12_3_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_B_8_5.jpg
Figure 3 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_7_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_7_3.jpg
Figure 4 Testis
(Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_4_6.jpg
(Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_4_6.jpg
Figure 5 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1887/159971_A_9_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (A549, CACO-2, SiHa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
Figure 6 RNA cell line category: Cell line enhanced (A549, CACO-2, SiHa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170458-CD14
Specifications
- Immunogen
- Purified human recombinant CD14
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human CD14
- Species Reactivity
- Human, Monkey
- Clone
- 28C5
- Applications
- ELISA, Flow Cytometry, Blocking
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Blocking: 28C5 antibody strongly inhibits LPS/LBP binding to sCD14 at 4 μg/mL.
Flow Cytometry: 10 μg/mL
ELISA: 1 μg/mL
Target
- Alternative Names
- CD14 Molecule; CD14 Antigen; Myeloid Cell-Specific Leucine-Rich Glycoprotein
- Gene ID
- 929
- UniProt ID
- P08571
- Long Name
- CD14 Molecule
- Cellular Localization
- Cell membrane; Golgi apparatus; Membrane; Secreted
- Post Translation Modifications
- N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
- Protein Refseq
- NP_000582.1; NP_001035110.1; NP_001167575.1
- Function
- Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655).
In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655, PubMed:22265692).
Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135).
Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211).
Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).
Recommended Products
Isotype Control
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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