Anti-Human CD14 Recombinant Antibody (Hm2F9) (CAT#: TAB-1588CL)
This antibody that specifically binds to CD14 with good positive cells without recognition of other organizations can be used in antiendotoxin therapy.
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FC
Figure 1 Flow cytometry analysis of Hm2F9 fusion.
Eight groups of PBMCs, including background control (A), isotype control (B), positive control parental murine antibody 2F9-FITC (C), culture supernatant from CHO-pCk plus GAH-Fc-FITC (D), culture supernatant from CHO-Hm2F9 plus GAH-Fc-FITC (E), 2F9 plus GAM-Fab-FITC (F), culture supernatant from CHO-pCk plus GAM-Fab-FITC (G), and culture supernatant from CHO-Hm2F9 plus GAM-Fab-FITC (H); three groups of negative control cell line (NALM-6), including isotype control (I), parental murine antibody 2F9-FITC (J), and culture supernatant from CHO-Hm2F9 (K). Two parameters as percentage of positive cells (%Gate) and mean fluorescence intensity (MFI Geo mean) were used to assess the binding activity of the Hm2F9 antibody. CHO, Chinese hamster ovary; FITC, fluorescein isothiocyanate; MFI, mean fluorescence intensity; PBMCs, peripheral blood mononuclear cells.
Shen, D. Y., Ning, B. T., Tang, Y. M., & Li, S. S. (2014). Construction and Expression of a Novel Anti-CD14 Human-Mouse Chimeric Antibody Hm2F9. DNA and cell biology, 33(9), 599-604.
FC
Figure 2 Biological function of Hm2F9 detected by flow cytometric analysis.
Four groups, including negative control (A), isotype group (B), LPS-FITC group (C), and Hm2F9 + LPS-FITC group (D), were designed. LPS, lipopolysaccharide; PBS, phosphate-buffered saline.
Shen, D. Y., Ning, B. T., Tang, Y. M., & Li, S. S. (2014). Construction and Expression of a Novel Anti-CD14 Human-Mouse Chimeric Antibody Hm2F9. DNA and cell biology, 33(9), 599-604.
Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric antibody (mouse/human)
- Specificity
- Human
- Clone
- Hm2F9
- Applications
- FC
- Related Disease
- Antiendotoxin therapy
Applications
- Application Notes
- Flow cytometry
Specific binding of Hm2F9 to CD14 was determined by flow cytometric analysis. Human peripheral blood mononuclear cells (PBMCs) expressing CD14 antigen and a negative control line, NALM-6, were used. Approximately 5 × 10⁵ cells were added to each tube and incubated with Hm2F9 and then bound protein was detected with fluorescein-labeled goat anti-human IgG Fc antibody and fluorescein-labeled goat anti-mouse IgG Fab (KPL). The fluorescent signals were analyzed by flow cytometry (FCM) using the CELLQUEST software.
Target
- Alternative Names
- CD14; CD14 molecule; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
- Gene ID
- 929
- UniProt ID
- P08571
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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