Anti-Human CD200R1 Recombinant Antibody (huDX182) (CAT#: TAB-1712CL)

Figure 1 shows the effects of anti-human CD200R antibodies DX176, DX177, DX178, DX182 and DX184 in a mast cell degranulation assay.

Figure 2 shows expression of CD200R on cultured cynomolgus mast cells using DX182 (anti-huCD200R).

Cultured peripheral blood derived cynomolgus mast cells were stained with control antibody, DX49 (isotype control for in vivo study), CD117 (stem cell factor receptor), DX182 (anti-huCD200R) and DX80 (FcεRI).

Figure 3 shows DX182 inhibits cultured cynomolgus mast cell FcεR1 induced degranulation.

Cultured cynomolgus mast cells were incubated with isotype control antibody, DX49 or anti-huCD200R antibody, DX182 and then triggered with an anti-FcεR1 antibody to induce degranulation. In a dose dependent fashion, DX182 inhibited the mast cell degranulation induced by triggering the FcεR1 receptor.

Figure 4 shows cynoCD200RLa is a single polypeptide chain that pairs with Dap 12.

Mouse mast cells transfected with cynomolgus CD200RLa were immunprecipitated with control antibody or DX182. DX182 immunoprecipitated cynomolgus CD200RLa from these transfectants. Western blotting for Dap12 shows that cynomolgus CD200RLa is associated with this signal adaptor protein.

Figure 5 shows DX182 induces degranulation of mouse mast cells expressing Cynomolgus CD200RLa.

Mouse mast cells transfected with cynomolgus CD200RLa were incubated with DX182 and assayed for mast cell degranulation. DX182 induced a potent mast cell degranulation.