Rat Anti-CD38 Recombinant Antibody (clone NIMR-5) (CAT#: NEUT-349CQ)
This antibody is a rat monoclonal antibody that binds specifically to mouse CD38, and it can neutralize the bioactivity of mouse CD38.
Specific Inquiry
SDS-PAGE
Figure 1 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in SDS-PAGE
SDS-PAGE analysis of NEUT-349CQ-mIgG1 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in SEC-HPLC
The purity of NEUT-349CQ-mIgG1 was greater than 98% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in DB
Dot Blot analysis of NEUT-349CQ-mIgG1 was performed by coating with mouse CD38 protein (His tag).
NEUT-349CQ-mIgG1 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG Fc as a secondary antibody (1: 2000)
ELISA
Figure 4 Rat Anti-CD38 Recombinant Antibody (NEUT-349CQ) in ELISA
ELISA analysis of NEUT-349CQ-mIgG1 was performed by coating with mouse CD38 protein (His tag). Then blocked with BSA and incubated with NEUT-349CQ-mIgG1. The HRP conjugated Anti-Mouse IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- BCL1 plasma membrane glycoproteins
- Host Species
- Rat
- Type
- Rat IgG2a, κ
- Specificity
- Recognizes Mouse CD38
- Species Reactivity
- Mouse
- Clone
- NIMR-5
- Applications
- Neut, FC, IHC-Fr, IP, BA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A or G purified
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD38; CD38 molecule; T10; ADPRC 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; cADPr hydrolase 1; CD38 antigen (p45); NAD(+) nucleosidase; ADP-ribosyl cyclase 1; cyclic ADP-ribose hydrolase 1; 2-phospho-ADP-ribosyl cyclase; 2-phospho-cyclic-ADP-ribose transferase; 2-phospho-ADP-ribosyl cyclase/2-phospho-cyclic-ADP-ribose transferase
- Gene ID
- 12494
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-079 | Recombinant Anti-Human CD38 VHH Single Domain Antibody | WB, IHC, FC, FuncS | Llama VHH |
| HPAB-0625-CN | Recombinant Bactrian camel Anti-CD38 Single Domain Antibody (HPAB-0625-CN) | ELISA | VHH |
| HPAB-0626-CN | Recombinant Bactrian camel Anti-CD38 Single Domain Antibody (HPAB-0626-CN) | ELISA | VHH |
Customer Reviews and Q&As
Customer Review
Q&As
Reliable for IHC Staining
This antibody reliably yields clear and specific staining results when used for immunohistochemistry. It's a dependable option for evaluating tissue segment CD38 expression.
Excellent Customer Support
This product has had outstanding customer support. My queries and worries have always been swiftly answered, which has made the whole process easy.
A Must-Have for CD38 Research
In my lab, this antibody has become essential for any CD38-related research. It is now a necessary tool for my studies because of its effectiveness and dependability.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NEUT-349CQ, RRID: AB_3111568)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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