Anti-Human CDCP1 Recombinant Antibody (25A11) (CAT#: TAB-403LC)

Figure 1 FACS profile of chimeric 25Al 1 IgG on PC-3, DuI 45 cell lines.

LN met is lymph node metastasis. GFI is the geometric mean of fluorescence intensity.

Figure 2 IHC staining of 25Al 1 on 1 : PC-3 cells, secondary alone (negative control), 2: PC-3 cells (positive control), 3: Patient 2, benign glands, 4: Patient 2, malignant glands, 5: Patient 5, benign glands, 6: Patient 5, malignant glands.

Figure 3 Summary of cell migration and invasion assays. PC-3 cells were treated with titrations of ch25Al 1 or CUBl, or 2.5 μM of PP2 and tested in Boyden chambers for inhibition of cell migration and invasion.

Figure 4 shows the internalization assay using anti-CDCPl antibodies with appropriate saporin secondary conjugates, or with ch25Al 1-Sap direct conjugate.

Figure 5 Effect of ch25Al 1 -saporin direct conjugate on PC-3 tumor growth.

Figure 1 Anti-Human CDCP1 Recombinant Antibody (TAB-403LC) in SDS-PAGE

SDS-PAGE analysis of TAB-403LC in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions.

Figure 2 Anti-Human CDCP1 Recombinant Antibody (TAB-403LC) in WB

Western blot analysis was performed with 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human CDCP1 onto a 12% Tris-HCl polyacrylamide gel. Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:5,000 for one hour.

Figure 3 Anti-Human CDCP1 Recombinant Antibody (TAB-403LC) in Dot Blot

Dot Blot analysis of TAB-403LC was performed with human CDCP1 antigen.
TAB-403LC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

Figure 4 Anti-Human CDCP1 Recombinant Antibody (TAB-403LC) in ELISA

ELISA analysis of TAB-403LC was performed by coating with recombinant human CDCP1 antigen. The HRP-conjugated goat anti-Mouse IgG was used as a secondary antibody.