Hi-Affi™ Rabbit Anti-CENPA Recombinant Antibody (clone DS661AB) (CAT#: MOR-0661)
Recombinant Rabbit anti-CENPA monoclonal antibody for FC, ICC/IF, IHC-P, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1443_A7_2_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1443_A7_2_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1494_A2_2_red_green.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1494_A2_2_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1772_D3_4_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1772_D3_4_red_green.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_7_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_7_3.jpg
Figure 5 Testis
(Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_4_6.jpg
(Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_4_6.jpg
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Eccrine glands
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Lymphocytes
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_B_8_1.jpg
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Eccrine glands
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Lymphocytes
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_B_8_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
Specifications
- Immunogen
- Synthetic peptide corresponding to residues in the C-terminal of human CENPA.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human CENPA
- Species Reactivity
- Human
- Clone
- DS661AB
- Applications
- FC, ICC, IF, IHC-P, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Centromere Protein A 2; Centromere-Specific Histone; Centromere Protein A, 17kDa; Centromere Autoantigen A; CENP-A; Histone H3-Like Centromeric Protein A; Centromere Protein A (17kD); CenH3
- Gene ID
- 1058
- UniProt ID
- P49450
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
Neutralizing Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1328z | Mouse Anti-CENPA Recombinant Antibody (clone 27A10) | WB, ICC, IF, IHC, Neut | Mouse IgG1 |
| MOB-0003CT | Recombinant Mouse anti-Human CENPA Monoclonal antibody (4-20) | ChIP, FC, ICC/IF, IHC-P, WB | |
| VS3-FY370 | Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5) | WB | Rabbit IgG |
| VS13-YC210 | Rabbit Anti-CENPA Recombinant Antibody (VS13-YC210) | WB, ICC, IF | Rabbit IgG |
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