Rabbit Anti-CENPA Recombinant Antibody (VS13-YC210) (CAT#: VS13-YC210)
The Rabbit Anti-CENPA Recombinant Antibody is specific for human CENPA. CENPA, a centromere protein, is critical for chromosome behavior during mitosis and gene regulation, affecting nucleosomal structure. The anti-CENPA antibody can be used for Western blot (1/500-1/2000, Immunofluorescence of cells (1/100).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1443_A7_2_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1443_A7_2_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1494_A2_2_red_green.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1494_A2_2_red_green.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1772_D3_4_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1772_D3_4_red_green.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_7_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_7_3.jpg
Figure 5 Testis
(Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_4_6.jpg
(Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_4_6.jpg
Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Eccrine glands
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Lymphocytes
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_B_8_1.jpg
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Eccrine glands
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Lymphocytes
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_B_8_1.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_8_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
Specifications
- Immunogen
- Recombinant human CENPA, aa 21-70 / 140.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CENPA
- Species Reactivity
- Human
- Applications
- WB, ICC, IF
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 40% Glycerol and 0.1% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunofluorescence (IF-cell): 1/100
Target
- Alternative Names
- Centromere Protein A; Histone H3-Like Centromeric Protein A; Centromere-Specific Histone; Centromere Protein A, 17kDa; Centromere Autoantigen A; CENP-A; Centromere Protein A (17kD); CenH3;
- Gene ID
- 1058
- UniProt ID
- P49450
- Long Name
- Centromere Protein A
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1328z | Mouse Anti-CENPA Recombinant Antibody (clone 27A10) | WB, ICC, IF, IHC, Neut | Mouse IgG1 |
| MOB-0003CT | Recombinant Mouse anti-Human CENPA Monoclonal antibody (4-20) | ChIP, FC, ICC/IF, IHC-P, WB | |
| VS3-FY370 | Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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