Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5)
CAT#: VS3-FY370
This product is a recombinant rabbit antibody that recognizes human CENPA protein. It can detect a band of approximately 16 kDa (observedd molecular weight: 16 kDa).
Tested Data
Gene Expression
WB
Figure 1. Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5) in WB.
Western blot analysis of CENPA in HeLa lysate K562 using CENPA Antibody.
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Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1443_A7_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1494_A2_2_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1772_D3_4_red_green.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_4_6.jpg
Normal Tissue
Figure 6 Skin
Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Eccrine glands
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Lymphocytes
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_B_8_1.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
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Specifications
- Immunogen
- A synthetic peptide of human CENPA
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CENPA
- Species Reactivity
- Human
- Clone
- R04-9A5
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 16 kDa; Observed MW: 16 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CENPA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1328z | Mouse Anti-CENPA Recombinant Antibody (clone 27A10) | WB, ICC, IF, IHC, Neut | Mouse IgG1 |
| MOB-0003CT | Recombinant Mouse anti-Human CENPA Monoclonal antibody (4-20) | ChIP, FC, ICC, IF, IHC-P, WB | |
| VS13-YC210 | Rabbit Anti-CENPA Recombinant Antibody (VS13-YC210) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-455CQ | Mouse Anti-CENPA Recombinant Antibody (clone 3-19) | ICC, IF, IHC, IHC-P, Neut, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0661 | Hi-Affi™ Rabbit Anti-CENPA Recombinant Antibody (clone DS661AB) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
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