Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5) (CAT#: VS3-FY370)

Name: Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5)
SKU: VS3-FY370
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes human CENPA protein. It can detect a band of approximately 16 kDa (observedd molecular weight: 16 kDa).

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5) in WB.

Figure 1. Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5) in WB.

Western blot analysis of CENPA in HeLa lysate K562 using CENPA Antibody.

Subcellular Location

Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1443_A7_2_selected.jpg

Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1494_A2_2_red_green.jpg

Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1772_D3_4_red_green.jpg

Normal Tissue

Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_7_3.jpg

Figure 5 Testis
(Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_4_6.jpg

Figure 6 Skin
(Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Eccrine glands
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Lymphocytes
Staining: Medium
Intensity: Moderate)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_B_8_1.jpg

Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_8_8.jpg

RNA Expression

Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthetic peptide of human CENPA

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human CENPA

Species Reactivity
Human

Clone
R04-9A5

Applications
Western Blot

Conjugate
Unconjugated

MW
Calculated MW: 16 kDa; Observed MW: 16 kDa

Product Property

Purification
Affinity Purified

Purity
>95%

Format
Liquid

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000

Target

Alternative Names
Centromere Protein A; Histone H3-Like Centromeric Protein A; Centromere-Specific Histone; Centromere Protein A, 17kDa; Centromere Autoantigen A; CENP-A; Centromere Protein A (17kD); CenH3

Gene ID
1058

UniProt ID
P49450

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CENPA"

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IgG Antibodies Neutralizing Antibodies Rabbit Monoclonal Antibodies

CAT	Product Name	Application	Type
MOB-1328z	Mouse Anti-CENPA Recombinant Antibody (clone 27A10)	WB, ICC, IF, IHC, Neut	Mouse IgG1
MOB-0003CT	Recombinant Mouse anti-Human CENPA Monoclonal antibody (4-20)	ChIP, FC, ICC, IF, IHC-P, WB
VS13-YC210	Rabbit Anti-CENPA Recombinant Antibody (VS13-YC210)	WB, ICC, IF	Rabbit IgG

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