Recombinant Mouse anti-Human CENPA Monoclonal antibody (4-20)
CAT#: MOB-0003CT
Recombinant Mouse monoclonal antibody to Human CENPA, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1443_A7_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1494_A2_2_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73086/1772_D3_4_red_green.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_4_6.jpg
Normal Tissue
Figure 6 Skin
Cells in basal layer
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in granular layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Cells in spinous layer
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Eccrine glands
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Langerhans cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Lymphocytes
Staining: Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_B_8_1.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8371/20852_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115163-CENPA
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Specifications
- Immunogen
- Synthetic peptide: PRRRSRKPEAPRRRSPS, corresponding to amino acids 4-20 of Human CENP A.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 4-20
- Applications
- Used for immunoassay techniques such as: ChImmunoprecipitation; Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein A purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None Constituents: 50% Glycerol, PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Target
- Alternative Names
- CENPA; centromere protein A; CenH3; CENP-A; histone H3-like centromeric protein A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone;
- Gene ID
- 1058
- UniProt ID
- P49450
- Protein Refseq
- NP_001035891
- Function
- DNA binding; chromatin binding; protein binding;
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1328z | Mouse Anti-CENPA Recombinant Antibody (clone 27A10) | WB, ICC, IF, IHC, Neut | Mouse IgG1 |
| VS3-FY370 | Recombinant Rabbit Anti-CENPA Antibody (clone R04-9A5) | WB | Rabbit IgG |
| VS13-YC210 | Rabbit Anti-CENPA Recombinant Antibody (VS13-YC210) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-455CQ | Mouse Anti-CENPA Recombinant Antibody (clone 3-19) | ICC, IF, IHC, IHC-P, Neut, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0661 | Hi-Affi™ Rabbit Anti-CENPA Recombinant Antibody (clone DS661AB) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
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