Recombinant Anti-Mouse Ctnnb1 VHH Single Domain Antibody

Name: Recombinant Anti-Mouse Ctnnb1 VHH Single Domain Antibody
SKU: NABG-049

CAT#: NABG-049

Anti-Mouse Ctnnb1 VHH Single Domain Antibody is a recombinant protein produced in E. coli.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/if_selected.jpg

Normal Tissue Figure 2 IHC staining of human stomach

Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29159/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human colon

Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/108/ihc_selected.jpg

Normal Tissue Figure 4 IHC staining of human liver

Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1950/ihc_selected.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_B_8_5.jpg

Normal Tissue Figure 6 Colon

Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_3.jpg

Normal Tissue Figure 7 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_4.jpg

Normal Tissue Figure 8 Pancreas

Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_1_3.jpg

Normal Tissue Figure 9 Kidney

Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_9_5.jpg

Normal Tissue Figure 10 Testis

Sertoli cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29160/62939_A_4_6.jpg

RNA Expression Figure 11 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000168036-CTNNB1

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Specifications

Immunogen
Mouse catenin (cadherin associated protein), beta 1

Host Species
Llama

Type
Llama VHH

Species Reactivity
Mouse

Molecule Class
VHH Single Domain Antibody

Applications
Formats of immunological tests: Enzyme-linked Immunosorbent Assay; Immunohistochemistry; Flow Cytometry; Functional Studies

Product Property

Purification
Purified on Immobilized Metal Affinity Chromatography (IMAC).

Purity
>95%, by SDS-PAGE with Coomassie Brilliant Blue staining

Storage
Store at -20°C. Avoid multiple freeze/thaw cycles.

Target

Alternative Names
CTNNB1; catenin (cadherin associated protein), beta 1; Bfc; Mesc; Catnb; catenin beta-1; beta-catenin

Gene ID
12387

UniProt ID
Q02248

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Versatile Application Spectrum

This Llama-derived recombinant antibody has been useful across multiple applications, including ELISA, Flow Cytometry, and Functional Studies. Its binding to mouse catenin beta-1 is specific and strong. The IMAC purification ensures a high-quality product that performs consistently in our diverse experimental setups.

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Related Diseases

Cushing Syndrome

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NABG-049.pdf

MSDS

MSDS - NABG-049.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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