Recombinant Mouse Antibody (1A1D-2) Fab Fragment is specific to DENV E-DIII, expressed in Chinese Hamster Ovary cells (CHO).
Figure 1 Characteristics of DIII-specific MAbs used in this study
Sukupolvi-Petty, S., Austin, S. K., Purtha, W. E., Oliphant, T., Nybakken, G. E., Schlesinger, J. J., ... & Diamond, M. S. (2007). Type-and subcomplex-specific neutralizing antibodies against domain III of dengue virus type 2 envelope protein recognize adjacent epitopes. Journal of virology, 81(23), 12816-12826.
Figure 2 Flow cytometry histograms of mAbs binding to yeast expressing wild-type or mutant domain III.
The following antibodies were used as negative and positive staining controls, respectively, for each of the indicated yeast based on data from a prior publication ( Sukupolvi-Petty et al., 2007 ): wild-type, WNV E16 and 3H5-1; K305E, 1A1D-2 and 3H5-1; K307C, 1A1D-2 and 3H5-1; K310E, 1A1D-2 and 3H5-1; P384A, 3H5-1 and 5A2-7; G330D, 6B6-10 and 3H5-1. In each case, staining with 9F12 is shown in red. The data are representative of three independent experiments.
Rajamanonmani, R., Nkenfou, C., Clancy, P., Yau, Y. H., Shochat, S. G., Sukupolvi-Petty, S., ... & Lescar, J. (2009). On a mouse monoclonal antibody that neutralizes all four dengue virus serotypes. Journal of General Virology, 90(4), 799-809.
Figure 3 Fab 1A1D-2:DENV complex formation is temperature dependent.
(a) DENV with no antibody. (b) DENV incubated with Fab 1A1D-2 at room temperature showed few particles with bound Fab molecules. (c) DENV incubated with Fab 1A1D-2 at 37 °C showed that most particles were saturated with Fab molecules.
Lok, S. M., Kostyuchenko, V., Nybakken, G. E., Holdaway, H. A., Battisti, A. J., Sukupolvi-Petty, S., ... & Diamond, M. S. (2008). Binding of a neutralizing antibody to dengue virus alters the arrangement of surface glycoproteins. Nature structural & molecular biology, 15(3), 312.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-275 | Recombinant Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
MHH-275 | Recombinant Human Anti-DENV E-DIII Antibody | ELISA, WB, FC, FuncS | IgG |
PABC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody (1A1D-2) | WB, ELISA | IgG |
PABL-437 | Recombinant Mouse Anti-DENV EDIII Antibody (2H12) | ELISA, Neut | IgG |
MRO-1612CQ | Recombinant Human Anti-DENV E-DIII Antibody (DV10) | ELISA, Neut | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-275-F(E) | Recombinant Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
MHH-275-F(E) | Recombinant Human Anti-DENV E-DIII Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
PFBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody Fab Fragment (2H12) | ELISA, Neut | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-275-S(P) | Recombinant Anti-DENV E-DIII Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
MHH-275-S(P) | Recombinant Human Anti-DENV E-DIII Antibody scFv Fragment | Neut, IF, FuncS | scFv |
PSBC-031 | Recombinant Mouse Anti-DENV E-DIII Antibody scFv Fragment (1A1D-2) | WB, ELISA | scFv |
PSBL-434 | Recombinant Mouse Anti-DENV EDIII Antibody scFv Fragment (2H12) | ELISA, Neut | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-044 | Recombinant Mouse Anti-DENV Antibody (3E31) | WB, ELISA, FuncS, Neut, Inhibt | IgG |
PABX-045 | Recombinant Mouse Anti-DENV Antibody (4E11) | WB, ELISA, FuncS, IF | IgG |
PABX-054 | Recombinant Mouse Anti-DENV Antibody (E111) | WB, ELISA, FuncS, SPR, Neut | IgG |
PABX-044-F (E) | Recombinant Mouse Anti-DENV Antibody Fab Fragment (3E31) | WB, ELISA, FuncS | Fab |
PABX-054-F (E) | Recombinant Mouse Anti-DENV Antibody Fab Fragment (E111) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-054-S (P) | Recombinant Mouse Anti-DENV Antibody scFv Fragment (E111) | WB, ELISA, FuncS | scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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