Hi-Affi™ Rabbit Anti-DOT1L Recombinant Antibody (clone DS1037AB)
CAT#: MOR-1037
Recombinant Rabbit anti-DOT1L monoclonal antibody for WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11875/1201_C5_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71217/1400_C9_1_selected.jpg
Normal Tissue
Figure 3 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate positivity in subset of cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_6_6_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_B_9_5.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74977/166292_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104885-DOT1L
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Specifications
- Immunogen
- Synthetic peptide corresponding to residues in Human KMT4/ Dot1L.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human DOT1L
- Species Reactivity
- Human
- Clone
- DS1037AB
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- DOT1-Like Histone H3K79 Methyltransferase; Histone H3-K79 Methyltransferase; Histone Methyltransferase DOT1L; Lysine N-Methyltransferase 4; DOT1-Like Protein; H3-K79-HMTase; KMT4; DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae); DOT1-Like, Histone H3 Methyltransferase; EC 2.1.1.43; KIAA1814; DOT1
- Gene ID
- 84444
- UniProt ID
- Q8TEK3
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "DOT1L"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1042CT | Recombinant Mouse anti-Human DOT1L Monoclonal antibody (EML1954) | FC, IP, WB | |
| ZG-0639J | Rabbit Anti-DOT1L Recombinant Antibody (clone 3B6) | ELISA, WB | Rabbit IgG |
| VS3-FY461 | Recombinant Rabbit Anti-DOT1L Antibody (clone R01-7F6) | WB, IHC-P | Rabbit IgG |
| VS-0625-FY1452 | Human Anti-DOT1L Recombinant Antibody (clone 2506W1347) | ELISA, IP MS | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2057 | Anti-DOT1L Immunohistochemistry Kit | IHC |
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