Recombinant Mouse Anti-EV71 VP1 Antibody (MA28-7) (CAT#: PABL-090)

Figure 2 NF449 inhibits immunoprecipitation of EV-A71-1095 by MA28-7 but not by 10F0.

EV-A71-1095 concentrated from supernatants of infected cells was incubated with MA28-7 or 10F0 fixed to Protein G beads, in the presence of NF449 at the indicated concentrations, or 200 μM Pirodavir; beads were washed, and immunoprecipitated proteins were examined on Coomassie-stained gels. Molecular weight markers are shown at the left. Arrows at the right indicate capsid proteins VP0 and VP1, and brackets indicate antibody heavy (HC) and light chains (LC).

Nishimura, Y., McLaughlin, N. P., Pan, J., Goldstein, S., Hafenstein, S., Shimizu, H.,... & Bergelson, J. M. (2015). The suramin derivative NF449 interacts with the 5-fold vertex of the enterovirus A71 capsid to prevent virus attachment to PSGL-1 and heparan sulfate. PLoS pathogens, 11(10), e1005184.

Figure 3 NF449 and NF110 inhibit attachment of MA28-7 to mature virions. Purified 35S-labeled mature virions were incubated with MA28-7-coated beads, in the presence of inhibitors as indicated.

Nishimura, Y., McLaughlin, N. P., Pan, J., Goldstein, S., Hafenstein, S., Shimizu, H.,... & Bergelson, J. M. (2015). The suramin derivative NF449 interacts with the 5-fold vertex of the enterovirus A71 capsid to prevent virus attachment to PSGL-1 and heparan sulfate. PLoS pathogens, 11(10), e1005184.