Recombinant Mouse Anti-FMDV-C-S8c1 VP1 Antibody (SD6) (CAT#: PABW-047)

Figure 1 Neutralization of FMDV as a function of the antibody SD6-to-virion input ratio.

Totals of 5×105 PFU(A), 2×106 PFU (B), or 6×107 PFU (C) of FMDV C-S8c1 (31) were mixed with different amounts of purified MAb SD6, the mixtures were incubated for 1 h at room temperature, and infectivity was determined in a plaque reduction assay as previously described (20). The ratios of physical-to-infectious particles for FMDV C-S8c1 in purified or partially purified FMDV preparations were determined by quantitating the virus titers in infectivity assays and by quantitating the amounts of viral proteins by SDS-PAGE and densitometry. These ratios ranged from 6,000 to 12,000 virion/PFU. These values are similar to those previously reported for other FMDVs (2).

Verdaguer, N., Fita, I., Domingo, E., & Mateu, M. G. (1997). Efficient neutralization of foot-and-mouth disease virus by monovalent antibody binding. Journal of virology, 71(12), 9813-9816.