Recombinant Anti-human GLI3 Intrabody [Tat] (CAT#: IAB-B069(T))

Recombinant anti-GLI3 intrabody in fusion with HIV Tat works within cells to bind specific to the repressor domain of human GLI3, expressed in E. coli cells. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Normal Tissue
RNA Expression

Specifications

  • Immunogen
  • human GLI family zinc finger 3
  • Host Species
  • llama
  • Type
  • vhh-Tat
  • Cell Penetrating Peptide
  • TAT
    TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
  • Species Reactivity
  • human
  • Applications
  • Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies

Product Property

  • Purity
  • >95% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE
  • Storage
  • Short Term Storage: 4°C
    Long Term Storage: -20°C

Target

  • Alternative Names
  • GLI3; GLI family zinc finger 3; PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190; transcriptional activator GLI3; oncogene GLI3; zinc finger protein GLI3; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3

Related Resources

  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "GLI3"

Fab Fragment Antibody

scFv Fragment Antibody

CAT Product Name Application Type
MOB-396-S(P) Recombinant Anti-human GLI3 Antibody scFv Fragment ELISA, WB, FuncS scFv
MHH-396-S(P) Recombinant Human Anti-human GLI3 Antibody scFv Fragment FC, RIA, Biosensors, FuncS scFv

Intrabody

CAT Product Name Application Type
IAB-B069(A) Recombinant Anti-human GLI3 Intrabody [(D-Arg)9] IF, FC, WB, FuncS vhh-(D-Arg)9
IAB-B069(G) Recombinant Anti-human GLI3 Intrabody [+36 GFP] ICC, Neut, FuncS vhh-(+36GFP)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1452 Hi-Affi™ Rabbit Anti-GLI3 Recombinant Antibody (clone DS1452AB) ICC, IF, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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