Recombinant Anti-human GLI3 Intrabody [Tat]
CAT#: IAB-B069(T)
Recombinant anti-GLI3 intrabody in fusion with HIV Tat works within cells to bind specific to the repressor domain of human GLI3, expressed in E. coli cells. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/61_A4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/1975_H4_2_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/59_A4_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_B_7_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (AF22, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106571-GLI3
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Specifications
- Immunogen
- human GLI family zinc finger 3
- Host Species
- llama
- Type
- vhh-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies
Product Property
- Purity
- >95% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE
- Storage
- Short Term Storage: 4°C
Long Term Storage: -20°C
Target
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Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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See other products for "GLI3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396 | Recombinant Anti-human GLI3 Antibody | WB, IP, FuncS | IgG |
| MHH-396 | Recombinant Human Anti-human GLI3 Antibody | ELISA, WB, Neut, FuncS | IgG |
| MOB-0773CT | Recombinant Mouse anti-Human GLI3 Monoclonal antibody (EML508) | WB | |
| MRO-0659-CN | Rabbit Anti-GLI3 Recombinant Antibody (clone JG83-39) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396-F(E) | Recombinant Anti-human GLI3 Antibody Fab Fragment | FC, IP, FuncS | Fab |
| MHH-396-F(E) | Recombinant Human Anti-human GLI3 Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396-S(P) | Recombinant Anti-human GLI3 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-396-S(P) | Recombinant Human Anti-human GLI3 Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B069(A) | Recombinant Anti-human GLI3 Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | vhh-(D-Arg)9 |
| IAB-B069(G) | Recombinant Anti-human GLI3 Intrabody [+36 GFP] | ICC, Neut, FuncS | vhh-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1452 | Hi-Affi™ Rabbit Anti-GLI3 Recombinant Antibody (clone DS1452AB) | ICC, IF, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY922 | Anti-GLI3 Immunohistochemistry Kit | IHC |
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