Hi-Affi™ Rabbit Anti-GLI3 Recombinant Antibody (clone DS1452AB)
CAT#: MOR-1452
Recombinant Rabbit anti-GLI3 monoclonal antibody for ICC/IF, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/61_A4_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/1975_H4_2_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/59_A4_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_B_7_5.jpg
Normal Tissue
Figure 6 Testis
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5534/16128_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (AF22, WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106571-GLI3
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Specifications
- Immunogen
- Recombinant fragment within Human Gli3 aa 1300-1500.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human GLI3
- Species Reactivity
- Human, Predicted to work with: Chimpanzee
- Clone
- DS1452AB
- Applications
- ICC, IF, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- GLI Family Zinc Finger 3; Glioma-Associated Oncogene Family Zinc Finger 3; GLI-Kruppel Family Member GLI3; Zinc Finger Protein GLI3; Oncogene GLI3; GLI3-190; GLI3FL; Greig Cephalopolysyndactyly Syndrome; GLI3 Full Length Protein; GLI3 Form Of 190 KDa; DNA-Binding Protein; PAP-A; PAPA1; PPDIV; ACLS; PAPA; PAPB; GCPS; PHS
- Gene ID
- 2737
- UniProt ID
- P10071
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Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "GLI3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396 | Recombinant Anti-human GLI3 Antibody | WB, IP, FuncS | IgG |
| MHH-396 | Recombinant Human Anti-human GLI3 Antibody | ELISA, WB, Neut, FuncS | IgG |
| MOB-0773CT | Recombinant Mouse anti-Human GLI3 Monoclonal antibody (EML508) | WB | |
| MRO-0659-CN | Rabbit Anti-GLI3 Recombinant Antibody (clone JG83-39) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396-F(E) | Recombinant Anti-human GLI3 Antibody Fab Fragment | FC, IP, FuncS | Fab |
| MHH-396-F(E) | Recombinant Human Anti-human GLI3 Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-396-S(P) | Recombinant Anti-human GLI3 Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| MHH-396-S(P) | Recombinant Human Anti-human GLI3 Antibody scFv Fragment | FC, RIA, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B069(A) | Recombinant Anti-human GLI3 Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | vhh-(D-Arg)9 |
| IAB-B069(G) | Recombinant Anti-human GLI3 Intrabody [+36 GFP] | ICC, Neut, FuncS | vhh-(+36GFP) |
| IAB-B069(T) | Recombinant Anti-human GLI3 Intrabody [Tat] | ELISA, WB, ICC, FuncS | vhh-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY922 | Anti-GLI3 Immunohistochemistry Kit | IHC |
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