Anti-GLI3 Intrabody [(D-Arg)9] (IAB-B069(A))
Recombinant anti-GLI3 intrabody linked to (D-Arg)9 works within cells to bind specific to the repressor domain of human GLI3, expressed in E. coli cells. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.
Specifications
- Immunogen
- human GLI family zinc finger 3
- Host
- llama
- Type
- vhh-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Immunofluorescence; Flow Cytometry; Western blot; Functional studies
Target
- Alternative Names
- GLI3; GLI family zinc finger 3; PHS; ACLS; GCPS; PAPA; PAPB; PAP-A
- Entrez Gene ID
- 2737
- UniProt ID
- P10071
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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