Anti-Human GM-CSF (Granulocyte-macrophage colony stimulating growth factor) Recombinant Antibody (F1) (CAT#: TAB-180CT)

Figure 1 Neutralization by mAbs against GM-CSF of bioactivity of GM-CSF.

Washed TF-1 cells were incubated with titrations of the indicated mAbs and 200 pg/mL rhGM-CSF. After 4 d, metabolic activity of TF-1 cells was measured by the water-soluble tetrazolium assay.

Wang, Y., Thomson, C. A., Allan, L. L., Jackson, L. M., Olson, M., Hercus, T. R., ... & Waddell, T. K. (2013). Characterization of pathogenic human monoclonal autoantibodies against GM-CSF. Proceedings of the National Academy of Sciences, 110(19), 7832-7837.

Figure 2 Neutralization by mAbs against GM-CSF of bioactivity of GM-CSF.

mAb F1 inhibits CD11b expression level on primary neutrophils stimulated by GM-CSF. Titrations of F1 mAb were added to heparinized blood with a high concentration of GM-CSF (10 ng/mL).

Wang, Y., Thomson, C. A., Allan, L. L., Jackson, L. M., Olson, M., Hercus, T. R., ... & Waddell, T. K. (2013). Characterization of pathogenic human monoclonal autoantibodies against GM-CSF. Proceedings of the National Academy of Sciences, 110(19), 7832-7837.

Figure 3 Neutralization of the bioactivity of GM-CSF by mAbs against GM-CSF.

After incubation with GM-CSF and titrations of mAb F1, PE-labeled anti-CD11b antibodies were added to the blood and held on ice. The cells were washed, and the neutrophils were gated on high side-scatter. Shown are histograms of fluorescence with different concentrations of mAb F1. Positive controls were GM-CSF (10 ng/mL) with no F1, and negative controls were neutrophils with no GM-CSF and F1 (300 ng/mL).

Wang, Y., Thomson, C. A., Allan, L. L., Jackson, L. M., Olson, M., Hercus, T. R., ... & Waddell, T. K. (2013). Characterization of pathogenic human monoclonal autoantibodies against GM-CSF. Proceedings of the National Academy of Sciences, 110(19), 7832-7837.