Recombinant Anti-HCV E2 VHH Single Domain Antibody (D03) (CAT#: PNBL-019)

Anti-HCV E2 VHH Single Domain Antibody is a recombinant protein produced in E. coli.

  • Published Data
  • Datasheet
  • MSDS
  • COA
FuncS

Figure 1 Neutralization activity of nanobodies.

The neutralizing ability of each nanobody (10 lg/mL) was determined using 100 focus-forming units of JFH-1 particles before inoculating Huh7.5 target cells. Neutralization was compared with control neutralizing mAb 1:7 and an irrelevant antitetanus toxin nanobody.

Tarr, A. W., Lafaye, P., Meredith, L., Damier‐Piolle, L., Urbanowicz, R. A., Meola, A., ... & Ball, J. K. (2013). An alpaca nanobody inhibits hepatitis C virus entry and cell‐to‐cell transmission. Hepatology, 58(3), 932-939.

Inhib

Figure 2 D03 inhibits cell-to-cell transmission of HCV

Transmission between HCV JFH-1–infected Huh-7.5 cells and na€ıve Huh-7.5 cells in the presence of inhibitors was assessed via flow cytometry. Extracellular virus after 24-hour incubation was quantified via end-point titration, and a horizontal black line indicates the number of infected target cells in the presence of an antibody dose that eliminated>95% of cell-free virus. Inhibition of cell-to-cell transmission was defined as reduced infection below this threshold. D03 demonstrated dose-dependent inhibition of direct infection.

Tarr, A. W., Lafaye, P., Meredith, L., Damier‐Piolle, L., Urbanowicz, R. A., Meola, A., ... & Ball, J. K. (2013). An alpaca nanobody inhibits hepatitis C virus entry and cell‐to‐cell transmission. Hepatology, 58(3), 932-939.

Figure 3 Genotype 1 clones show both resistant and sensitive phenotypes.

HCVpp supplemented with genotypes were neutralized by increasing concentrations of mAb nB D03 and the resulting infectivity plotted against antibody concentration.

Urbanowicz, R. A., McClure, C. P., Brown, R. J., Tsoleridis, T., Persson, M. A., Krey, T., ... & Tarr, A. W. (2016). A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance. Journal of virology, 90(7), 3288-3301.

Figure 4 Normally distributed IC50 values for each antibody.

Frequency distribution plots of log10 IC50 values, and the derived Gaussian curve, from all 81 clones for mAb D03. Mean log10 IC50 value for each antibody is represented by a red dashed line, mean ± 2 standard deviations by a solid red line and mean ± 3 standard deviations by the grey fill. Each plot fitted a normal distribution curve as shown by the D'Agostino & Pearson omnibus normality test.

Urbanowicz, R. A., McClure, C. P., Brown, R. J., Tsoleridis, T., Persson, M. A., Krey, T., ... & Tarr, A. W. (2016). A diverse panel of hepatitis C virus glycoproteins for use in vaccine research reveals extremes of monoclonal antibody neutralization resistance. Journal of virology, 90(7), 3288-3301.


Specifications

  • Immunogen
  • HCV E2 envelope protein
  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • HCV
  • Clone
  • D03
  • Applications
  • Functional Assay, Inhibition

Applications

  • Application Notes
  • The antibody was validated for Functional Assay, Inhibition. For details, refer to Published Data.

Target

  • Alternative Names
  • E2; HCV; envelope protein; hepatitis C virus

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "E2"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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