Human Anti-HGFAC Recombinant Antibody (clone Ab58) (CAT#: PABZ-058)
Recombinant Human Antibody (Ab58) is capable of binding to HGFAC, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HGFAC mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HGFAC mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. Ab58 is a competitive inhibitor.
Specific Inquiry
Figure 1 Inhibition of HGFA catalytic activity by Ab58 and Ab75.
(A and B) Cleavage of ¹²⁵I-pro-HGF by HGFA in the presence of 3-fold serial dilutions of Ab58 (A) and Ab75 (B). The cleavage products HGF α- and β-chain were analyzed by SDS/PAGE (reducing conditions) and x-ray film exposure. C, control (no antibody). The last lane in B contained ¹²⁵I-pro-HGF only. (C) Partial inhibition of Spectrozyme FVIIa hydrolysis (expressed as HGFA fractional activity vᵢ/vo) by Ab75 and complete inhibition by Ab58 (Inset). (D) Eadie– Hofstee plot of HGFA inhibition by Ab75 (2–0.008 M in 3-fold dilution steps; filled circles, ''no antibody'' control) shows competitive inhibition. Vmax = 0.99 μM pNA/min and Km = 0.24 mM for control; Vmaxapp = 1.00 M pNA/min and Kmapp = 1.21 mM for 2 μM Ab75 (triangles).
Wu, Y., Eigenbrot, C., Liang, W. C., Stawicki, S., Shia, S., Fan, B., ... & Kirchhofer, D. (2007). Structural insight into distinct mechanisms of protease inhibition by antibodies. Proceedings of the National Academy of Sciences, 104(50), 19784-19789.
Figure 2 Competition binding ELISA measuring binding of HGFA to biotinylated KD1 in the presence of increasing antibody concentrations.
Wu, Y., Eigenbrot, C., Liang, W. C., Stawicki, S., Shia, S., Fan, B., ... & Kirchhofer, D. (2007). Structural insight into distinct mechanisms of protease inhibition by antibodies. Proceedings of the National Academy of Sciences, 104(50), 19784-19789.
Specifications
- Immunogen
- Human HGF activator
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human HGFAC
- Species Reactivity
- Human
- Clone
- Ab58
- Applications
- ELISA, Block
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody Ab58 has been reported in applications of Inhib, ELISA, Block. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- HGFAC; HGF activator; HGFA; hepatocyte growth factor activator
- Gene ID
- 3083
- UniProt ID
- D6RAR4
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone Ab58"
See other products for "HGFAC"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-463 | Recombinant Anti-human HGFAC Antibody | WB, ELISA, IHC, FuncS | IgG |
| MHH-463 | Recombinant Human Anti-human HGFAC Antibody | Neut, WB, FuncS | IgG |
| PABL-133 | Recombinant Human Anti-HGFAC Antibody (Ab40) | WB, ELISA, FuncS | IgG |
| PABW-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab75) | ELISA, Block | Human IgG |
| HPAB-0003-SJF | Human Anti-HGFAC Recombinant Antibody (HPAB-0003-SJF) | ELISA, FuncS | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-463-F(E) | Recombinant Anti-human HGFAC Antibody Fab Fragment | FC, WB, FuncS | Fab |
| PFBL-133 | Recombinant Human Anti-HGFAC Antibody Fab Fragment (Ab40) | WB, ELISA, FuncS | Fab |
| PFBZ-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab58); Fab Fragment | ELISA, Block | Human Fab |
| PFBW-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab75); Fab Fragment | ELISA, Block | Human Fab |
| HPAB-0003-SJF-F(E) | Human Anti-HGFAC Recombinant Antibody; Fab Fragment (HPAB-0003-SJF-F(E)) | ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-463-S(P) | Recombinant Anti-human HGFAC Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| MHH-463-S(P) | Recombinant Human Anti-human HGFAC Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| HPAB-S0059-YC-S(P) | Human Anti-HGFAC Recombinant Antibody (clone Ab39); scFv Fragment | ELISA | Human scFv |
| HPAB-S0060-YC-S(P) | Human Anti-HGFAC Recombinant Antibody (clone Ab40); scFv Fragment | ELISA, Inhib | Human scFv |
| HPAB-0003-SJF-S(P) | Human Anti-HGFAC Recombinant Antibody; scFv Fragment (HPAB-0003-SJF-S(P)) | ELISA, FuncS | Human scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-079CT | Human Anti-HGFAC Recombinant Antibody (TAB-079CT) | ELISA | Humanized antibody |
| TAB-079CT-S(P) | Human Anti-HGFAC Recombinant Antibody; scFv Fragment (TAB-079CT-S(P)) | ELISA | Humanized scFv |
| TAB-079CT-F(E) | Human Anti-HGFAC Recombinant Antibody; Fab Fragment (TAB-079CT-F(E)) | ELISA | Humanized Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-083 | Recombinant Human Anti-HGFA Antibody (Fab40.deltaTrp) | WB, ELISA, Neut, FuncS | IgG |
| PABX-083-S (P) | Recombinant Human Anti-HGFA Antibody scFv Fragment (Fab40.deltaTrp) | WB, ELISA, Neut, FuncS | scFv |
| PABX-083-F (E) | Recombinant Human Anti-HGFA Antibody Fab Fragment (Fab40.deltaTrp) | WB, ELISA, Neut, FuncS | Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABZ-058. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.