Recombinant monoclonal antibody 6A9 to hydroxylated hypoxia inducible factor 1-α (HIF-1α). The isotype is mouse IgG2a. The antibody has been tested in western blot (1/200) with cell Lysates and Immunofluorescence (1/100) assays. This antibody intended for the prophylaxis and treatment studies of Pancreatic cancer, Colorectal cancer.
Figure 1 Validation of monoclonal antibodies against hydroxylated proline 402 or 564 containing peptides of the HIF-1α ODD region.
Clone 6A9 was specific for hydroxylated proline 564.
Rolfo, A., Many, A., Racano, A., Tal, R., Tagliaferro, A., Ietta, F., ... & Caniggia, I. (2010). Abnormalities in oxygen sensing define early and late onset preeclampsia as distinct pathologies. PloS one, 5(10), e13288.
Figure 2 HIF-1α hydroxylation during normal placentation and in preeclampsia.
Representative immunoblots showing reduced HIF-1a hydroxylation at P564 (6A9) in preeclamptic placentae (E-PE, n = 12) relative to preterm controls (PTC n = 12).
Rolfo, A., Many, A., Racano, A., Tal, R., Tagliaferro, A., Ietta, F., ... & Caniggia, I. (2010). Abnormalities in oxygen sensing define early and late onset preeclampsia as distinct pathologies. PloS one, 5(10), e13288.
Figure 3 SUMO2/3-mediated SUMOylation results in decreased HIF1A stability.
Western blot showing reduced HIF1A hydroxylation at P564 (Clone 6A9) in JEG3 cells exposed to 3% O2 relative to 21% O2 standard conditions.
Bhattacharjee, J., Alahari, S., Sallais, J., Tagliaferro, A., Post, M., & Caniggia, I. (2016). Dynamic regulation of HIF1Α stability by SUMO2/3 and SENP3 in the human placenta. Placenta, 40, 8-17.
Figure 1 Analysis of mAb reactivity with hCD200R by orientation via antibody immobilization.
Shows the mean fluorescence intensity ± SEM for each spot of all replicates. Serial two-fold dilutions of capture human CD200R mAb OX108, DX136 and DX147 and control rat CD4 mAb OX68 were arrayed onto epoxy-coated microscope slides. Each mAb dilution series was arrayed in quadruplicate of 2 rows of 6 spots, ranging in concentration from 80 μg ml-1 (first spot) to 0.16 μg/ml (spot 10), with control spotting buffer containing 0.5 mg/ml BSA in the last two spots.
LETARTE, Michelle, et al. Analysis of leukocyte membrane protein interactions using protein microarrays. BMC biochemistry, 2005, 6.1: 2.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1828z | Mouse Anti-HIF1A Recombinant Antibody (clone 6F11) | WB, ELISA, IF | Mouse IgG1 |
MOB-0903CT | Recombinant Mouse anti-Human HIF1A Monoclonal antibody (2B4) | ELISA, ICC/IF, IHC-P, WB | |
VS3-XY804 | Mouse Anti-HIF1A Recombinant Antibody (clone 5B1) | IHC | |
VS3-XY805 | Mouse Anti-HIF1A Recombinant Antibody (clone 1A3) | ELISA, WB, IHC, ICC | Mouse IgG1 |
VS3-WK1354 | Mouse Anti-HIF1A Recombinant Antibody (VS3-WK1354) | WB, IHC | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-080CT-F(E) | Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (6A9) | WB, Inhibit | |
TAB-081CT-F(E) | Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (1H1) | WB, Inhibit | |
TAB-082CT-F(E) | Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (6H4) | WB, Inhibit | |
TAB-083CT-F(E) | Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (5A5) | WB, Inhibit | |
TAB-084CT-F(E) | Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (7D6) | WB, Inhibit |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1595 | Rabbit Anti-HIF1A Recombinant Antibody (clone DS1595AB) | IHC, WB, IHC-P | Rabbit IgG |
MOR-4589 | Rabbit Anti-HIF1A Recombinant Antibody (clone TH102DS) | WB, IF, ICC, FC, ChIP, ELISA | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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