Mouse Anti-NHP HIF1A Recombinant Antibody (clone H1alpha67)
CAT#: VS-1024-XY248
Designed for accurate detection of HIF1A, this mouse monoclonal antibody exhibits broad reactivity with species including bovine, cat, ferret, human, mouse, non-human primate, sheep, rabbit, and rat. The immunogen consists of a fusion protein, ensuring strong antigen recognition. Validated for Western blot (WB), immunohistochemistry (IHC), immunoprecipitation (IP), and ELISA, this antibody provides versatility and reliability in studying HIF1A expression and function across various experimental applications and species.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
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Specifications
- Immunogen
- Fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- HIF1A
- Species Reactivity
- Bovine, Cat, Ferret, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat
- Clone
- H1alpha67
- Applications
- WB, IHC, IP, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500, IHC: 1:20-1:50, IP: 1:10-1:500, ELISA: 1:100-1:1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "HIF1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1828z | Mouse Anti-HIF1A Recombinant Antibody (clone 6F11) | WB, ELISA, IF | Mouse IgG1 |
| MRO-1928-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1928-CN) | WB, IF, FC | Rabbit IgG |
| MRO-1929-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1929-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-QX510 | Mouse Anti-HIF1A Recombinant Antibody (clone 5H7) | WB, IP, ICC, IHC | Mouse IgG1 |
| VS7-0425-WR34 | Mouse Anti-HIF1A Monoclonal Antibody (VS7-0425-WR34) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-080CT | Anti-Human HIF-1alpha Recombinant Antibody (6A9) | FuncS | |
| TAB-081CT | Anti-Human HIF-1alpha Recombinant Antibody (1H1) | WB | |
| TAB-082CT | Anti-Human HIF-1alpha Recombinant Antibody (6H4) | WB | |
| TAB-083CT | Anti-Human HIF-1alpha Recombinant Antibody (5A5) | WB, Inhibit | |
| TAB-084CT | Anti-Human HIF-1alpha Recombinant Antibody (7D6) | WB, Inhibit |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1595 | Rabbit Anti-HIF1A Recombinant Antibody (clone DS1595AB) | IHC, WB, IHC-P | Rabbit IgG |
| MOR-4589 | Rabbit Anti-HIF1A Recombinant Antibody (clone TH102DS) | WB, IF, ICC, FC, ChIP, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3136 | Anti-HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3137 | Anti-Mouse HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3138 | Anti-Porcine HIF1A Immunohistochemistry Kit | IHC | |
| VS-0525-XY3139 | Anti-Rabbit HIF1A Immunohistochemistry Kit | IHC |
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