Anti-Human HIF-1alpha Recombinant Antibody (6H4) (CAT#: TAB-082CT)

Name: Anti-Human HIF-1alpha Recombinant Antibody (6H4)
SKU: TAB-082CT
Rating: 5 (1 reviews)

Recombinant monoclonal antibody (6H4) to HIF-1alpha. The isotype is mouse IgG1. The antibody has been tested in western blot (1/100) with cell Lysates and Immunofluorescence (1/100) assays. This antibody intended for the prophylaxis and treatment studies of Pancreatic cancer, Colorectal cancer.

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Published Data
Gene Expression
Datasheet
MSDS
COA

Figure 1 Validation of monoclonal antibodies against hydroxylated proline 402 or 564 containing peptides of the HIF-1α ODD region.

Figure 1 Validation of monoclonal antibodies against hydroxylated proline 402 or 564 containing peptides of the HIF-1α ODD region.

Clones 6H4 recognized specifically HIF-1α hydroxylated at proline 402 as demonstrated by Western blotting of lysates of JEG-3 cells transfected with a HIF-1α single or double mutated construct at P402 and/or P564.

Rolfo, A., Many, A., Racano, A., Tal, R., Tagliaferro, A., Ietta, F., ... & Caniggia, I. (2010). Abnormalities in oxygen sensing define early and late onset preeclampsia as distinct pathologies. PloS one, 5(10), e13288.

Normal Tissue

Figure 1 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg

Figure 2 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg

Figure 3 Cerebral cortex
(Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg

Figure 4 Gallbladder
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg

Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg

RNA Expression

Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Host Species
Mouse

Specificity
Human

Species Reactivity
Human

Clone
6H4

Applications
WB

Related Disease
Pancreatic cancer, Colorectal cancer

Applications

Application Notes
This antibody has been reported for use in Western Blot.

Target

Alternative Names
HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha

Gene ID
3091

UniProt ID
Q16665

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT	Product Name	Application	Type
MOB-1828z	Mouse Anti-HIF1A Recombinant Antibody (clone 6F11)	WB, ELISA, IF	Mouse IgG1
MOB-0903CT	Recombinant Mouse anti-Human HIF1A Monoclonal antibody (2B4)	ELISA, ICC, IF, IHC-P, WB
ZG-0430J	Mouse Anti-HIF1A Recombinant Antibody (ZG-0430J)	WB, IHC, IF, ELISA	Mouse IgG
ZG-0038U	Rabbit Anti-HIF1A Recombinant Antibody (clone 5D12)	ELISA, IF	Rabbit IgG
VS3-QX510	Mouse Anti-HIF1A Recombinant Antibody (clone 5H7)	WB, IP, ICC, IHC	Mouse IgG1

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CAT	Product Name	Application
TAB-080CT-F(E)	Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (6A9)	WB, Inhibit
TAB-081CT-F(E)	Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (1H1)	WB, Inhibit
TAB-082CT-F(E)	Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (6H4)	WB, Inhibit
TAB-083CT-F(E)	Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (5A5)	WB, Inhibit
TAB-084CT-F(E)	Anti-Human HIF-1alpha Recombinant Antibody Fab Fragment (7D6)	WB, Inhibit

CAT	Product Name	Application	Type
MOR-1595	Rabbit Anti-HIF1A Recombinant Antibody (clone DS1595AB)	IHC, WB, IHC-P	Rabbit IgG
MOR-4589	Rabbit Anti-HIF1A Recombinant Antibody (clone TH102DS)	WB, IF, ICC, FC, ChIP, ELISA	Rabbit IgG

CAT	Product Name	Application
VS-0525-XY3136	Anti-HIF1A Immunohistochemistry Kit	IHC
VS-0525-XY3137	Anti-Mouse HIF1A Immunohistochemistry Kit	IHC
VS-0525-XY3138	Anti-Porcine HIF1A Immunohistochemistry Kit	IHC
VS-0525-XY3139	Anti-Rabbit HIF1A Immunohistochemistry Kit	IHC