SmartAb™ Recombinant Anti-HIF1A pH-dependent Antibody (Clone 1H1)
CAT#: VS-0825-YC156
The anti-HIF1A pH-dependent antibody (clone 1H1) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human HIF-1alpha. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for HIF-1alpha under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from HIF-1alpha at pH 6.0. This pH-sensitive antibody retains its binding affinity for HIF-1alpha on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human HIF-1alpha, or Hypoxia-Inducible Factor-1 alpha, is a transcription factor that is a master regulator of the cellular response to low oxygen levels (hypoxia). It regulates the expression of genes involved in angiogenesis, metabolism, and cell survival.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17442/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_B_8_5.jpg
Normal Tissue
Figure 4 Gallbladder
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1275/3828_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (LHCN-M2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100644-HIF1A
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a kappa
- Specificity
- Human HIF-1alpha
- Species Reactivity
- Human
- Clone
- 1H1
- Applications
- WB
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- Product Name
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| CAT | Product Name | Application | Type |
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| TAB-080CT | Anti-Human HIF-1alpha Recombinant Antibody (6A9) | FuncS |
| CAT | Product Name | Application | Type |
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| TAB-081CT | Anti-Human HIF-1alpha Recombinant Antibody (1H1) | WB |
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| TAB-082CT | Anti-Human HIF-1alpha Recombinant Antibody (6H4) | WB |
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| TAB-083CT | Anti-Human HIF-1alpha Recombinant Antibody (5A5) | WB, Inhibit |
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| MOB-0903CT | Recombinant Mouse anti-Human HIF1A Monoclonal antibody (2B4) | ELISA, ICC, IF, IHC-P, WB |
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| MOR-1595 | Rabbit Anti-HIF1A Recombinant Antibody (clone DS1595AB) | IHC, WB, IHC-P | Rabbit IgG |
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| MOR-4589 | Rabbit Anti-HIF1A Recombinant Antibody (clone TH102DS) | WB, IF, ICC, FC, ChIP, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-1928-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1928-CN) | WB, IF, FC | Rabbit IgG |
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| MRO-1929-CN | Rabbit Anti-HIF1A Polyclonal Antibody (MRO-1929-CN) | IF, IHC, FC | Rabbit IgG |
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| ZG-0430J | Mouse Anti-HIF1A Recombinant Antibody (ZG-0430J) | WB, IHC, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-1024-XY248 | Mouse Anti-NHP HIF1A Recombinant Antibody (clone H1alpha67) | WB, IHC, IP, ELISA | Mouse IgG2b |
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| VS-0525-XY3136 | Anti-HIF1A Immunohistochemistry Kit | IHC |
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| VS-0525-XY3137 | Anti-Mouse HIF1A Immunohistochemistry Kit | IHC |
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| VS-0525-XY3138 | Anti-Porcine HIF1A Immunohistochemistry Kit | IHC |
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| VS-0525-XY3139 | Anti-Rabbit HIF1A Immunohistochemistry Kit | IHC |
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