Anti-HIV-1 gp120 Recombinant Antibody (BAT123) (CAT#: MRO-132LC)

Figure 1 mAb-induced gpl20 dissociation.

The cells were pelleted, and the supernatant was spun through 0.22-/xm filters to remove cell debris and treated with NP-40, 1% final concentration. Gp120-mAb complexes in the supernatants were then denatured to prevent interference of gp120 detection by bound Ig, and gp120 concentration was measured by ELISA. Each bar represents the mean signal obtained from duplicate ELISA wells, and the dotted line represents the level of spontaneous gpl20 dissociation.

Human Immunodeficiency Virus Type 1 Neutralization Is Determined by Epitope Exposure on the gp120 Oligomer

Figure 2 Binding of neutralizing mAbs to recombinant and cell surface gpl20.

HIV-infected cells were stained for mAb binding by indirect immunofluorescence.

Human Immunodeficiency Virus Type 1 Neutralization Is Determined by Epitope Exposure on the gp120 Oligomer

Figure 3 mAb neutralization of HIV-1.

A stock of virus previously titered on HeLa-CD4-LTR LacZ cells and known to give 200 infected cells/well in 20/zl was incubated for 2 h with mAbs at the concentrations shown in the figure before washing and trypsinization to remove noninternalized virions. The cells were subsequently cultured for 24 h at 37~ before fixation and staining with X-Gal for 4 h at 37~ The cells were then fixed overnight, and the blue loci were counted by eye under magnification. Each point represents the mean of triplicate wells, and the vertical bars indicate +_ 1 SD.

Human Immunodeficiency Virus Type 1 Neutralization Is Determined by Epitope Exposure on the gp120 Oligomer

Figure 4 is a plot showing the relative effectiveness of four of the monoclonal antibodies of the invention in neutralizing HIV-1 infection of H9 cells.

The percentage of infected cells was determined thirteen days after infection.

Figure 5 Inhibitory activity of antibodies on sCD4-gpl2O binding. mAbs were G3-4 (v), 15e (A), G3-519 (o), and BAT123 (o).

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody

Figure 6 Inhibitory activity of mAbs G34 (A), BAT123 (o), G3-519 (e), and 15e (o) on the binding of biotinylated mAb G3-4 to captured 111B gpl20.

Another discontinuous epitope on glycoprotein gpl20 that is important in human immunodeficiency virus type 1 neutralization is identified by a monoclonal antibody