Recombinant Human Anti-HIV-1 gp120 Antibody (B13) (CAT#: PABW-153)
Recombinant Human Antibody (B13) is capable of binding to HIV-1 gp120, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp120 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-HIV-1 gp120 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. Unlike other CD4BS antibodies, antibody b13 showed substantial, though reduced, binding to the 109-428 variant. In addition, b13 was able to recognize an outer domain–only variant of gp120.
Specific Inquiry
ELISA
Figure 1 Effect of 17b on bNAb and non-bNAb binding to monomeric HXBc2 gp120 core by Sandwich ELISA assay.
Saturating amount of 17b was coated on ELISA plate and HXBc2 gp120 core was added to form complex with 17b, followed by the addition of biotinylated human MAbs (VRC01, PGV04, F105, b6, b13) and NHP MAbs (GE125, GE136 and GE140). Binding was detected with a streptavidin-HRP conjugate and the absorbance was read at 450 nm. OD, optical density.
Chen, Y., Wilson, R., O’Dell, S., Guenaga, J., Feng, Y., Tran, K., ... & Li, Y. (2016). An HIV-1 Env-antibody complex focuses antibody responses to conserved neutralizing epitopes. The Journal of Immunology, 197(10), 3982-3998.
ELISA
Figure 2 ELISA binding profiles of human CD4bs and non-CD4bs MAbs (left), and selected sera of Imm 3 and Imm 7 (right) from complex-GLA immunized rabbit group tested with CD4bs bNAb probes, RSC3/G367R and RSC3 Δ371I/P363N.
Chen, Y., Wilson, R., O’Dell, S., Guenaga, J., Feng, Y., Tran, K., ... & Li, Y. (2016). An HIV-1 Env-antibody complex focuses antibody responses to conserved neutralizing epitopes. The Journal of Immunology, 197(10), 3982-3998.
Figure 3 The binding kinetics of CD4bs MAb b13 and representative rabbit MAbs 30-9 and 46-1 to HXBc2 core and RSC3 core.
Different concentrations of the core were shown.
Chen, Y., Wilson, R., O’Dell, S., Guenaga, J., Feng, Y., Tran, K., ... & Li, Y. (2016). An HIV-1 Env-antibody complex focuses antibody responses to conserved neutralizing epitopes. The Journal of Immunology, 197(10), 3982-3998.
SPR
Figure 4 Surface plasmon resonance analysis
Surface plasmon resonance analysis of deglycosylated unlabeled, 15N and 15N/13C labeled binding to monoclonal antibodies b12 and b13 demonstrates that the expressed protein is correctly folded and biologically active.
Sastry, M., Xu, L., Georgiev, I. S., Bewley, C. A., Nabel, G. J., & Kwong, P. D. (2011). Mammalian production of an isotopically enriched outer domain of the HIV-1 gp120 glycoprotein for NMR spectroscopy. Journal of biomolecular NMR, 50(3), 197-207.
Specifications
- Immunogen
- HIV-1 gp120 Protein
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native HIV-1 gp120 protein
- Species Reactivity
- HIV-1
- Clone
- B13
- Applications
- Can be useful in applications such as: Neutralization, Flow Cytometry
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Immunotoxin
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MHC Tetramer for Virology
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L012R | anti-env immunotoxin 907 (IgG)-RTA | Cytotoxicity assay, Functional assay |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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