Recombinant Anti-HIV-1 gp120 VHH Single Domain Antibody (PNBL-059) (CAT#: PNBL-059)

Anti-HIV-1 gp120 VHH Single Domain Antibody is a recombinant protein produced in E. coli.

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ELISA

Figure 1 Dose-dependent competition of VHH A12, D7, and C8 with sCD4 for binding to recombinant envelope proteins in the ELISA.

Figure 1 Dose-dependent competition of VHH A12, D7, and C8 with sCD4 for binding to recombinant envelope proteins in the ELISA.

Threefold serial dilutions of VHH were preincubated with IIIB gp120 or 92UG037 gp140 and subsequently incubated with sCD4 precoated on microtiter plates. Envelope protein binding to sCD4 was detected as described in the text. Chemiluminescence was measured, and background subtracted luminescence readings (in relative light units [RLU]) were plotted against VHH concentration. Data points represent the means and bars show the standard deviations of duplicate reactions.

Forsman, A., Beirnaert, E., Aasa-Chapman, M. M., Hoorelbeke, B., Hijazi, K., Koh, W., ... & Verrips, T. (2008). Llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (HIV-1)-neutralizing properties and high affinity for HIV-1 gp120. Journal of virology, 82(24), 12069-12081.

Inhib

Figure 2 VHH and anti-gp120 MAb cross-competition analysis.

Figure 2 VHH and anti-gp120 MAb cross-competition analysis.

VHH competition with anti-gp120 MAbs 2G12 (carbohydrate motif), 17b (CD4i), and 447-52D (V3), as well as MAb b12 (CD4bs), for binding to recombinant gp120 in the ELISA. Wells of microtiter plates were coated with VHH A12, D7, and C8, as indicated above each graph. Serial dilutions of each MAb were preincubated with IIIB gp120 and subsequently incubated with the immobilized VHH.

Forsman, A., Beirnaert, E., Aasa-Chapman, M. M., Hoorelbeke, B., Hijazi, K., Koh, W., ... & Verrips, T. (2008). Llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (HIV-1)-neutralizing properties and high affinity for HIV-1 gp120. Journal of virology, 82(24), 12069-12081.

ELISA

Figure 3 VHH and anti-gp120 MAb cross-competition analysis.

Figure 3 VHH and anti-gp120 MAb cross-competition analysis.

VHH competition with each other and with sCD4 for binding to recombinant gp120 in the ELISA. Wells of microtiter plates were coated with VHH A12, D7, and C8, as indicated above each graph. Serial dilutions of each VHH and sCD4 were preincubated with IIIB gp120 and subsequently incubated with the immobilized VHH.

Forsman, A., Beirnaert, E., Aasa-Chapman, M. M., Hoorelbeke, B., Hijazi, K., Koh, W., ... & Verrips, T. (2008). Llama antibody fragments with cross-subtype human immunodeficiency virus type 1 (HIV-1)-neutralizing properties and high affinity for HIV-1 gp120. Journal of virology, 82(24), 12069-12081.


Specifications

  • Host Species
  • Llama
  • Derivation
  • Llama
  • Type
  • Llama VHH
  • Species Reactivity
  • HIV-1
  • Clone
  • PNBL-059
  • Applications
  • ELISA, Inhibition

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The antibody was validated for ELISA, Inhibition. For details, refer to Published Data.

Target

  • Alternative Names
  • ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160, precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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