Recombinant Human Anti-Hepatitis B virus Antibody (KR127) (CAT#: PABX-078)

Anti-Hepatitis B virus antibody is a Humanized antibody of IgG class that binds to an Hepatitis B virus. The humanized monoclonal antibody recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo.

Host: Human

Isotype: IgG

Applications: WB 1:1000-1:5000
ELISA 1:10000-1:20000
FuncS (assay dependent)


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Figure 1 Validation of epitope specificity with HzKR127-3.2.

GST-preS1 (amino acids 1–56) protein (WT) and a series of alanine replacement mutants were expressed in E. coli and subjected to 12% SDS-PAGE (bottom) followed by western blot analysis with KR127, HzKR127-3.1, or HzKR127-3.2 (Top). The protein band corresponding to the GST-preS1 is marked.

Kim, J. H., Gripon, P., Bouezzedine, F., Jeong, M. S., Chi, S. W., Ryu, S. E., & Hong, H. J. (2015). Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen–antibody complex structure. FEBS letters, 589(2), 193-200.

Figure 2 Validation of off-target activity with HzKR127-3.2.

HzKR127-3.2 was subjected to flow cytometric analysis using preS1-expressing HEK293T cells (293T-S1-L1) and preS1-negative cells (HEK293T, SCK-L1, ACHN, B16F1, and CHO).

Kim, J. H., Gripon, P., Bouezzedine, F., Jeong, M. S., Chi, S. W., Ryu, S. E., & Hong, H. J. (2015). Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen–antibody complex structure. FEBS letters, 589(2), 193-200.

Figure 3 Affinity determination of murine and humanized KR127 antibody by a competitive ELISA.

A solution containing 3 ng of the purified antibody and various concentrations (1011–106 M) of the GST-preS1 protein as a competing antigen was preincubated at 37 jC for 3 h and added to each well that was coated with 0.1 Ag of the GST-preS1, then an indirect ELISA was done. Apparent affinity was determined as the reciprocal of the antigen concentration required to inhibit 50% maximal binding in the competitive ELISA.

Hong, H. J., Ryu, C. J., Hur, H., Kim, S., Oh, H. K., Oh, M. S., & Park, S. Y. (2004). In vivo neutralization of hepatitis B virus infection by an anti-preS1 humanized antibody in chimpanzees. Virology, 318(1), 134-141.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Hepatitis B virus Hepatitis B virus
  • Species Reactivity
  • Hepatitis B virus
  • Clone
  • KR127
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:10000-1:20000
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • HBV; Hepatitis B virus; Hepatitis B

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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