Recombinant Human Anti-Hepatitis B virus Antibody scFv Fragment (KR127) (CAT#: PABX-078-S (P))
Anti-Hepatitis B virus antibody is a Human of scFv class that binds to an Hepatitis B virus. The humanized monoclonal antibody recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Validation of epitope specificity with HzKR127-3.2.
GST-preS1 (amino acids 1–56) protein (WT) and a series of alanine replacement mutants were expressed in E. coli and subjected to 12% SDS-PAGE (bottom) followed by western blot analysis with KR127, HzKR127-3.1, or HzKR127-3.2 (Top). The protein band corresponding to the GST-preS1 is marked.
Kim, J. H., Gripon, P., Bouezzedine, F., Jeong, M. S., Chi, S. W., Ryu, S. E., & Hong, H. J. (2015). Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen–antibody complex structure. FEBS letters, 589(2), 193-200.
Figure 2 Validation of off-target activity with HzKR127-3.2.
HzKR127-3.2 was subjected to flow cytometric analysis using preS1-expressing HEK293T cells (293T-S1-L1) and preS1-negative cells (HEK293T, SCK-L1, ACHN, B16F1, and CHO).
Kim, J. H., Gripon, P., Bouezzedine, F., Jeong, M. S., Chi, S. W., Ryu, S. E., & Hong, H. J. (2015). Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen–antibody complex structure. FEBS letters, 589(2), 193-200.
Figure 3 Affinity determination of murine and humanized KR127 antibody by a competitive ELISA.
A solution containing 3 ng of the purified antibody and various concentrations (1011–106 M) of the GST-preS1 protein as a competing antigen was preincubated at 37 jC for 3 h and added to each well that was coated with 0.1 Ag of the GST-preS1, then an indirect ELISA was done. Apparent affinity was determined as the reciprocal of the antigen concentration required to inhibit 50% maximal binding in the competitive ELISA.
Hong, H. J., Ryu, C. J., Hur, H., Kim, S., Oh, H. K., Oh, M. S., & Park, S. Y. (2004). In vivo neutralization of hepatitis B virus infection by an anti-preS1 humanized antibody in chimpanzees. Virology, 318(1), 134-141.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- scFv
- Specificity
- Tested positive against native Human NOR
- Species Reactivity
- Hepatitis B virus
- Clone
- KR127
- Applications
- WB 1:1000-1:5000
ELISA 1:3000-1:15000
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- HBV; Hepatitis B virus; Hepatitis B
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone KR127"
See other products for "HBV"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
NAB-1234-sdAb | Recombinant Anti-HBV VHH Single Domain Antibody | IHC, IP, FC, Neut, FUNC | Llama VHH |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for PABX-078-S (P). Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: Depletion, FuncS
Application: ELISA, FC, IHC, Neut
Application: ELISA, WB, Microarray, Block
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.