Recombinant Human Anti-Hepatitis B virus Antibody scFv Fragment (KR127)
CAT#: PABX-078-S (P)
Anti-Hepatitis B virus antibody is a Human of scFv class that binds to an Hepatitis B virus. The humanized monoclonal antibody recognizes the preS1 domain of the human hepatitis B virus surface proteins with a broadly neutralizing activity in vivo.
Published Data
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Figure 1 Validation of epitope specificity with HzKR127-3.2.
GST-preS1 (amino acids 1–56) protein (WT) and a series of alanine replacement mutants were expressed in E. coli and subjected to 12% SDS-PAGE (bottom) followed by western blot analysis with KR127, HzKR127-3.1, or HzKR127-3.2 (Top). The protein band corresponding to the GST-preS1 is marked.
Kim, J. H., Gripon, P., Bouezzedine, F., Jeong, M. S., Chi, S. W., Ryu, S. E., & Hong, H. J. (2015). Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen–antibody complex structure. FEBS letters, 589(2), 193-200.
-2.png)
Figure 2 Validation of off-target activity with HzKR127-3.2.
HzKR127-3.2 was subjected to flow cytometric analysis using preS1-expressing HEK293T cells (293T-S1-L1) and preS1-negative cells (HEK293T, SCK-L1, ACHN, B16F1, and CHO).
Kim, J. H., Gripon, P., Bouezzedine, F., Jeong, M. S., Chi, S. W., Ryu, S. E., & Hong, H. J. (2015). Enhanced humanization and affinity maturation of neutralizing anti-hepatitis B virus preS1 antibody based on antigen–antibody complex structure. FEBS letters, 589(2), 193-200.
-3.png)
Figure 3 Affinity determination of murine and humanized KR127 antibody by a competitive ELISA.
A solution containing 3 ng of the purified antibody and various concentrations (1011–106 M) of the GST-preS1 protein as a competing antigen was preincubated at 37 jC for 3 h and added to each well that was coated with 0.1 Ag of the GST-preS1, then an indirect ELISA was done. Apparent affinity was determined as the reciprocal of the antigen concentration required to inhibit 50% maximal binding in the competitive ELISA.
Hong, H. J., Ryu, C. J., Hur, H., Kim, S., Oh, H. K., Oh, M. S., & Park, S. Y. (2004). In vivo neutralization of hepatitis B virus infection by an anti-preS1 humanized antibody in chimpanzees. Virology, 318(1), 134-141.
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- scFv
- Specificity
- Tested positive against native Human NOR
- Species Reactivity
- Hepatitis B virus
- Clone
- KR127
- Applications
- WB 1:1000-1:5000
ELISA 1:3000-1:15000
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
- Alternative Names
- HBV; Hepatitis B virus; Hepatitis B
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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