Recombinant Mouse Anti-HBV Pres1 Region Antibody (5a19) (CAT#: PABL-113)

Recombinant Mouse Antibody (5a19) is capable of binding to HBV Pres1 Region , expressed in Chinese Hamster Ovary cells (CHO).

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Figure 1 Lymphocytes obtained from a healthy donor were retrovirally grafted with cTCR containing scFv recognizing HBV S protein (S-C8), HBV L protein (L-5a19), or carcinoembryonic antigen (CEA) or used as control (PBL). Flow cytometric analysis using a PE-conjugated anti-CD3 and an FITC-conjugated anti-human Ig-Fc antibody, which detects the extracellular IgG1 CH2CH3 spacer domain of the receptors, were performed to identify cTCR grafted T cells.

Bohne, F., Chmielewski, M., Ebert, G., Wiegmann, K., Kürschner, T., Schulze, A.,... & Protzer, U. (2008). T cells redirected against hepatitis B virus surface proteins eliminate infected hepatocytes. Gastroenterology, 134(1), 239-247.

Figure 2 Cytotoxic T-cell response and cytokine secretion.

T cells grafted with the cTCR directed either against HBV S (S-C8, open diamond) or L protein (L-5a19, open circle) or against CEA (CEA, solid square) or unmodified cells (PBL, solid rectangle) were cultured together with HBV (upper panel) HepG2.2.15 cells or HBV (lower panel) HepG2 cells. T cells were added in different dilutions to obtain indicated effector to target cell (E:T) ratios and cocultured for 72 hours. (A) Specific lysis of target cells in 3 parallel assays is shown. Secretion of IFN- (B) and IL-2 (C) into cell culture media was measured by ELISA. Mean  SD is given. (D) Antigen-specific proliferation was determined by flow cytometry of CFSE stained, cTCR grafted T cells after 48 hours. One representative staining (E:T 0.8:10) out of 4 stainings is shown.

Bohne, F., Chmielewski, M., Ebert, G., Wiegmann, K., Kürschner, T., Schulze, A.,... & Protzer, U. (2008). T cells redirected against hepatitis B virus surface proteins eliminate infected hepatocytes. Gastroenterology, 134(1), 239-247.

Figure 3 HBV-infected primary human hepatocytes are eliminated by antigen-specific, engineered T cells.

Primary human hepatocytes were infected with HBV and cultured for 3 days prior to the addition of redirected T cells (HBV target cells: black columns; HBV control cells: grey columns). T cells grafted with cTCR S-C8, L-5a19, or CEA, respectively, or unmodified cells (PBL) were added as an effector to target cell ratio of 2:1 and cocultured for 96 hours. (A) Liver transaminase (ALT) levels were determined in cell culture media as a marker for hepatocyte lysis. Mean values and standard deviations obtained from 3 independent infection experiments are given. For comparison, ALT levels of CD95-treated cells undergoing apoptosis are shown. (B) IFN- and (C) IL-2 were measured in the culture supernatant by ELISA. (D) HBeAg and (E) HBsAg were determined in cell culture supernatants by ELISA. (F) Western blot analysis of HBV-infected PHH cells for intracellular albumin and HBV core proteins. (G) HBV rcDNA and (H) HBV cccDNA were quantified by real-time PCR in cellular DNA preparations from infected hepatocyte cultures upon coculture with redirected T cells.

Bohne, F., Chmielewski, M., Ebert, G., Wiegmann, K., Kürschner, T., Schulze, A.,... & Protzer, U. (2008). T cells redirected against hepatitis B virus surface proteins eliminate infected hepatocytes. Gastroenterology, 134(1), 239-247.


Specifications

  • Immunogen
  • Hepatitis B virus
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • IgG
  • Specificity
  • Tested positive against native HBV HBV
  • Species Reactivity
  • HBV
  • Clone
  • 5a19
  • Applications
  • WB, ELISA, FuncS

Applications

  • Application Notes
  • The antibody was validated for FuncS. For details, refer to published data.

Target

  • Alternative Names
  • HBV; Hepatitis B virus; Hepatitis B

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "HBV"


For research use only. Not intended for any clinical use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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