Recombinant Mouse anti-Human MMP14 Monoclonal antibody (EML768)
CAT#: MOB-1237CT
Recombinant Mouse monoclonal antibody to Human MMP14, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/1591_G3_3_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong luminal membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/134761_A_8_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows positivity in stromal cells and plasma.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line BJ
Immunofluorescent staining of human cell line BJ shows localization to cytosol & intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51432/1658_G1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_8_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9918/24805_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC diff, HHSteC, HSkMC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000157227-MMP14
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Specifications
- Immunogen
- Purified human recombinant MMP14.
- Host Species
- Mouse
- Specificity
- It recognizes human MMP14 in western blot without cross reactivity with human MMP1-13, or MMP14-15. Reactivity with Ang1 of other species has not been tested.
- Species Reactivity
- Human
- Clone
- EML768
- Applications
- Used for immunoassay techniques such as: Immunohistochemistry paraffin embedded sections; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.2 mg/ml
- Buffer
- Preservative: None Constituents: PBS
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Target
- Alternative Names
- MMP14; matrix metallopeptidase 14 (membrane-inserted); MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase;
- Gene ID
- 4323
- UniProt ID
- P50281
- Protein Refseq
- NP_004986
- Function
- calcium ion binding; integrin binding; metalloendopeptidase activity; peptidase activator activity; protein binding; sequence-specific DNA binding transcription factor activity; zinc ion binding;
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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