Mouse Anti-MMP14 Recombinant Antibody (clone LEM-2/15) (CAT#: PABL-279)
Recombinant mouse antibody (LEM-2/15) is capable of binding to MT1-MMP. The antibody LEM-2/15 was generated by immunizing mice with a peptide possessing the sequence of the V-B loop (aa 218-233).
Specific Inquiry
Figure 1 Immunohistochemistry with the LEM2/15 anti MT1-MMP antibody in a TMA (n = 40) of Low-grade GBMs (LGG) and High-grade GBMs (HGG) patient tissues (representative images). Right panel: MT1-MMP staining intensity score (0–3).
de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.
Figure 2 Immunoblotting analyses of the indicated cell lines using the LEM2/15 anti MT1-MMP antibody.
de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.
Figure 3 FACS analyses of the indicated cell lines using the LEM2/15 anti MT1-MMP antibody.
de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.
Figure 4 Immunohistochemistry of tumor tissue from xenografted mice used for PET imaging. MT1-MMP was detected using LEM2/15 antibody. Scale bars: 500 μm.
de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.
Figure 5 PET/CT imaging with radiolabeled 89Zr-DFO-LEM2/15 in mice bearing orthotopic xenografts containing patient-derived neurospheres.
A) Representative fused PET/CT images of coronal and sagittal planes at 2 and 4 days p.i. containing TS543 brain tumors with 89Zr-DFO-LEM2/15 (left panels) and 89Zr-DFO-IgG1 as isotype control (right panels) B) Tumor-to-blood ratios for 89Zr-DFO-LEM2/15 (black bars) and 89Zr-DFO-IgG1 (grey bars) in nude mice bearing orthotopic TS453 xenografts at 2 and 4 days p.i. derived from PET imaging.
de Lucas, A. G., Schuhmacher, A. J., Oteo, M., Romero, E., Cámara, J. A., de Martino, A.,... & Mulero, F. (2016). Targeting MT1-MMP as an ImmunoPET-based strategy for imaging gliomas. PloS one, 11(7), e0158634.
Figure 6 ANS fluorescence assay. ANS (250 mM) was added to CAT-MT1-MMP (5 mM), Fab LEM-2/15 (5 mM), and a 1:1 complex. The samples were excited at 350 nm, and fluorescence spectra (400–600 nm) were recorded. The fluorescence intensity was calculated as the area of the spectra at 400–600 nm (ANS, black; MT1- MMP, red; Fab LEM-2/15, green; MT1-MMP:Fab LEM-2/15, blue).
The inset shows emission spectra of the proteins in the presence of ANS (ANS, black; MT1- MMP, red; Fab LEM-2/15, green; MT1-MMP:Fab LEM-2/15, blue).
Udi, Y., Grossman, M., Solomonov, I., Dym, O., Rozenberg, H., Moreno, V.,... & Sagi, I. (2015). Inhibition mechanism of membrane metalloprotease by an exosite-swiveling conformational antibody. Structure, 23(1), 104-115.
Specifications
- Immunogen
- A peptide possessing the sequence of the V-B loop, residues 218-233.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MMP14
- Species Reactivity
- Human
- Clone
- LEM-2/15
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- MMP14; matrix metallopeptidase 14 (membrane-inserted); MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase
- Gene ID
- 4323
- UniProt ID
- P50281
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-865-VHH | Recombinant Anti-human MMP14 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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