Human Anti-IL17RA Recombinant Antibody (TAB-134)
CAT#: TAB-134
This product is a recombinant Human antibody that recognizes IL17RA. The antibody was purified by affinity chromatography.
Tested Data
Gene Expression
ELISA
Figure 1 Anti-Human IL17RA Antibody (TAB-134) in ELISA
ELISA analysis of TAB-134 was performed by coating with Human IL17RA Protein (His Tag). Then blocked with BSA and incubated with TAB-134. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human IL17RA Antibody (TAB-134) in WB
Western blot analysis of TAB-134 was performed with Human IL17RA Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-134 (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-Human IL17RA Antibody (TAB-134) in DB
Dot Blot analysis of TAB-134 was performed by coating with Human IL17RA Protein (His Tag).
TAB-134 incubation concentration: 2 μg/mL.
HRP goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24996/if_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24996/59803_A_7_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24996/59803_A_7_4.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HL-60, NB-4, THP-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177663-IL17RA
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG2, κ
- Species Reactivity
- Human
- Applications
- Suitable for use in FuncS, IF, Neut, ELISA, FC, IP, ICC and most other immunological methods.
- MW
- 144.06 kDa
- Related Disease
- Psoriatic arthritis (PSA)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Customer Review
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Excellent for ELISA
I have used the human anti-IL17RA recombinant antibody for ELISA and immunofluorescence studies with great success. The product demonstrates excellent binding to IL17RA and provides consistent results that enhance the interpretation of receptor expression in my cell cultures.
Consistent Results
The recombinant IL17RA antibody meets my expectations in both functional studies and immunoprecipitations. Its high purity (>95% by SDS-PAGE) and the reliable performance in various applications such as IP, IF, and FC have made my receptor analyses more precise and informative.
Q&As
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Is this antibody suitable for ELISA and immunoprecipitation studies?
A: Yes, the recombinant human anti-IL17RA antibody has been validated for use in ELISA, immunoprecipitation, and other immunoassays such as IF and FC. Its high purity and specific binding to IL17RA make it an excellent candidate for detailed receptor studies in your lab research.
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What are the key features that affirm its specificity for IL17RA?
A: The antibody is produced recombinantly and exhibits excellent specificity to IL17RA, a type I membrane glycoprotein. Its purity exceeding 95% by SDS-PAGE and multiple application validations, including FC and ICC, ensure that it reliably binds IL17RA in various in vitro research settings.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-134, RRID: AB_3111829)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| HPAB-0319-WJ | Human Anti-IL17RA Recombinant Antibody (HPAB-0319-WJ) | ELISA, Neut | Humanized IgG |
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| CAT | Product Name | Application | Type |
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| HPAB-0320-WJ-S(P) | Human Anti-IL17RA Recombinant Antibody; scFv Fragment (HPAB-0320-WJ-S(P)) | ELISA, Neut | Humanized scFv |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-0322-WJ-S(P) | Human Anti-IL17RA Recombinant Antibody; scFv Fragment (HPAB-0322-WJ-S(P)) | ELISA, Neut | Humanized scFv |
| CAT | Product Name | Application | Type |
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| HPAB-0318-WJ-F(E) | Human Anti-IL17RA Recombinant Antibody; Fab Fragment (HPAB-0318-WJ-F(E)) | ELISA, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
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| HPAB-0319-WJ-F(E) | Human Anti-IL17RA Recombinant Antibody; Fab Fragment (HPAB-0319-WJ-F(E)) | ELISA, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
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| HPAB-0320-WJ-F(E) | Human Anti-IL17RA Recombinant Antibody; Fab Fragment (HPAB-0320-WJ-F(E)) | ELISA, Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
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| HPAB-0321-WJ-F(E) | Human Anti-IL17RA Recombinant Antibody; Fab Fragment (HPAB-0321-WJ-F(E)) | ELISA, Neut | Humanized Fab |
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| HPAB-0322-WJ-F(E) | Human Anti-IL17RA Recombinant Antibody; Fab Fragment (HPAB-0322-WJ-F(E)) | ELISA, Neut | Humanized Fab |
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