Mouse Anti-IL1RL1 Recombinant Antibody (TAB-222CT)

Name: Mouse Anti-IL1RL1 Recombinant Antibody (TAB-222CT)
SKU: TAB-222CT
Rating: 5 (1 reviews)

CAT#: TAB-222CT

This product is a recombinant mouse antibody that recognizes IL1RL1. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. It has been tested using Enzyme-linked immunosorbent assay, Inhibition assay and Flow cytometry.

Tested Data Gene Expression

Figure 1 Anti-IL1RL1 Recombinant Antibody (TAB-222CT) in WB

Western blot analysis of TAB-222CT was performed by loading Mouse IL1RL1 Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg, 0.3 μg, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-222CT (2 μg/mL) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.

Figure 2 Anti-IL1RL1 Recombinant Antibody (TAB-222CT) in DB

Dot Blot analysis of TAB-222CT was performed by coating with Mouse IL1RL1 Protein (His Tag).

TAB-222CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)

ELISA

Figure 3 Anti-IL1RL1 Recombinant Antibody (TAB-222CT) in ELISA

ELISA analysis of TAB-222CT was performed by coating with Mouse IL1RL1 Protein (His Tag). Then blocked with BSA and incubated with TAB-222CT. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane, cytosol & focal adhesion sites.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7406/44_B9_1_selected.jpg

Normal Tissue Figure 2 IHC staining of human placenta

Immunohistochemical staining of human placenta shows membranous and cytoplasmic positivity in trophoblastic cells and stromal cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7406/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7917/ihc_selected.jpg

Normal Tissue Figure 5 Colon

Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7406/23807_A_7_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7406/23807_A_8_4.jpg

Normal Tissue Figure 7 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7406/23807_A_8_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7406/23807_A_6_6.jpg

Normal Tissue Figure 10 Lymph node

Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7406/23807_A_9_8.jpg

RNA Expression Figure 11 RNA cell line category: Group enriched (HHSteC, HMC-1, HUVEC TERT2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115602-IL1RL1

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Specifications

Immunogen
Mouse ST2-Fc

Host Species
Mouse

Type
Mouse IgG1

Specificity
Mouse IL1RL1

Species Reactivity
Mouse

Applications
ELISA, Inhib, FC

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
See the COA

Buffer
PBS

Preservative
No preservatives

Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target

Alternative Names
T1; ST2; DER4; ST2L; ST2V; FIT-1; IL33R; interleukin-1 receptor-like 1; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; interleukin 1 receptor-related protein

Gene ID
17082

UniProt ID
P14719

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-222CT.pdf

MSDS

MSDS - TAB-222CT.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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