Human Anti-IL21 Recombinant Antibody (HPAB-0315-CN) (CAT#: HPAB-0315-CN)
This product is a recombinant antibody targeting and recognizing Interleukin 21, derived from human. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography. The antibody has potent affinity against IL21. It has been detected by Enzyme-linked immunosorbent assay and Neutralization assay.
Specific Inquiry
WB
Figure 1 Anti-IL21 Recombinant Antibody (HPAB-0315-CN) in WB
Western blot analysis of HPAB-0315-CN was performed by loading human IL21 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0315-CN and HRP goat anti-human IgG as a secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-IL21 Recombinant Antibody (HPAB-0315-CN) in DB
Dot Blot analysis of HPAB-0315-CN was performed by coating with human IL21 protein (His tag).
HPAB-0315-CN incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 3000)
ELISA
Figure 3 Anti-IL21 Recombinant Antibody (HPAB-0315-CN) in ELISA
ELISA analysis of HPAB-0315-CN was performed by coating with human IL21 protein (His tag). Then blocked with BSA and incubated with HPAB-0315-CN. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 4000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG1
- Specificity
- Human IL21
- Species Reactivity
- Human, Cynomolgus
- Applications
- ELISA, Neut
- Related Disease
- Graft-versus-host disease (GVHD)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "IL21"
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-415CL | Human Anti-IL21 Recombinant Antibody (TAB-415CL) | ELISA | Human IgG |
| TAB-572LC | Human Anti-IL21 Recombinant Antibody (TAB-572LC) | Inhib | Humanized IgG |
| TAB-573LC | Human Anti-IL21 Recombinant Antibody (TAB-573LC) | Inhib | Humanized IgG |
| TAB-574LC | Human Anti-IL21 Recombinant Antibody (TAB-574LC) | Inhib | Humanized IgG |
| TAB-576LC | Human Anti-IL21 Recombinant Antibody (TAB-576LC) | ELISA, FC, Neut, FuncS | Humanized antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-570LC | Mouse Anti-IL21 Recombinant Antibody (TAB-570LC) | ELISA, FC, Inhib | Mouse IgG |
| TAB-571LC | Mouse Anti-IL21 Recombinant Antibody (TAB-571LC) | ELISA, FC, Inhib | Mouse IgG |
| TAB-575LC | Mouse Anti-IL21 Recombinant Antibody (TAB-575LC) | ELISA, FC | Mouse IgG |
| TAB-570LC-S(P) | Mouse Anti-IL21 Recombinant Antibody; scFv Fragment (TAB-570LC-S(P)) | ELISA | Mouse scFv |
| TAB-571LC-S(P) | Mouse Anti-IL21 Recombinant Antibody; scFv Fragment (TAB-571LC-S(P)) | ELISA | Mouse scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-577LC-F(E) | Human Anti-IL21 Recombinant Antibody; Fab Fragment (TAB-577LC-F(E)) | ELISA, FC | Human Fab |
| TAB-578LC-F(E) | Human Anti-IL21 Recombinant Antibody; Fab Fragment (TAB-578LC-F(E)) | ELISA, WB, FC | Human Fab |
| TAB-579LC-F(E) | Human Anti-IL21 Recombinant Antibody; Fab Fragment (TAB-579LC-F(E)) | ELISA, WB, FC | Human Fab |
| TAB-580LC-F(E) | Human Anti-IL21 Recombinant Antibody; Fab Fragment (TAB-580LC-F(E)) | ELISA, WB, FC | Human Fab |
| TAB-581LC-F(E) | Human Anti-IL21 Recombinant Antibody; Fab Fragment (TAB-581LC-F(E)) | ELISA, WB, FC | Human Fab |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0291MZ | Chicken Anti-Interleukin-21 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1325CQ | Mouse Anti-IL21 Recombinant Antibody (clone MT216G/21.3m) | Neut | Mouse IgG1 |
| NEUT-1326CQ | Rat Anti-Il21 Recombinant Antibody (clone FFA21) | FuncS, Neut, IF | Rat IgG2a, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1802 | Rabbit Anti-IL21 Recombinant Antibody (clone DS1802AB) | WB | Rabbit IgG |
| MOR-4260 | Rabbit Anti-IL21 Recombinant Antibody (clone SI340DS) | ELISA | Rabbit IgG |
| MOR-4613 | Rabbit Anti-IL21 Recombinant Antibody (clone TH126DS) | ELISA | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0315-CN-S(P) | Human Anti-IL21 Recombinant Antibody; scFv Fragment (HPAB-0315-CN-S(P)) | ELISA, Neut | Human scFv |
| HPAB-0657-CN-S(P) | Mouse Anti-IL21 Recombinant Antibody (clone 3E11); scFv Fragment | WB, ELISA, IF, FC, Neut | Mouse scFv |
| HPAB-0287-FY-S(P) | Human Anti-IL21 Recombinant Antibody (clone mAb14); scFv Fragment | Inhib, Neut | Human scFv |
| FAMAB-0210WJ-S(P) | Mouse Anti-IL21 Recombinant Antibody (clone 9H10); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0211WJ-S(P) | Mouse Anti-IL21 Recombinant Antibody (clone 8B6); scFv Fragment | ELISA, WB | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0287-FY | Human Anti-IL21 Recombinant Antibody (clone mAb14) | Inhib, Neut | Human IgG4, κ |
| HPAB-0130-WJ | Human Anti-IL21 Recombinant Antibody (HPAB-0130-WJ) | FC, IHC | Humanized IgG |
| MOB-Z003-YC | Mouse Anti-IL21 Recombinant Antibody (clone DEC.YC003) | ELISA, WB | Mouse IgG1, κ |
| FAMAB-0210WJ | Mouse Anti-IL21 Recombinant Antibody (clone 9H10) | ELISA, WB | Mouse IgG |
| FAMAB-0211WJ | Mouse Anti-IL21 Recombinant Antibody (clone 8B6) | ELISA, WB | Mouse IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0287-FY-F(E) | Human Anti-IL21 Recombinant Antibody (clone mAb14); Fab Fragment | Inhib, Neut | Human Fab, κ |
| HPAB-M0538-YC-F(E) | Mouse Anti-IL21 Recombinant Antibody; Fab Fragment (HPAB-M0538-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-M0539-YC-F(E) | Mouse Anti-IL21 Recombinant Antibody; Fab Fragment (HPAB-M0539-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-M0540-YC-F(E) | Mouse Anti-IL21 Recombinant Antibody; Fab Fragment (HPAB-M0540-YC-F(E)) | ELISA | Mouse Fab |
| FAMAB-0210WJ-F(E) | Mouse Anti-IL21 Recombinant Antibody (clone 9H10); Fab Fragment | ELISA, WB | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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