Human Anti-JAG1 Recombinant Antibody (NS-049CN)
CAT#: NS-049CN
This product is a recombinant human antibody that recognizes JAG1. The immunogen is the human Jag1-DSL-EGF1-4. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21555/48721_A_8_5_selected.jpg
Normal Tissue
Figure 3 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/25743_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/25743_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/25743_A_8_4.jpg
Normal Tissue
Figure 7 Gallbladder
Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/25743_A_6_4.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/25743_A_5_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10343/25743_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HBEC3-KT, hTERT-HME1, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101384-JAG1
❮
❯
Specifications
- Immunogen
- Human Jag1-DSL-EGF1-4
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG
- Specificity
- Human JAG1
- Species Reactivity
- Human
- Applications
- ELISA, WB, IF
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| HPAB-0943LY-F(E) | Human Anti-JAG1 Recombinant Antibody; Fab Fragment (HPAB-0943LY-F(E)) | ELISA | Human Fab |
| HPAB-0944LY-F(E) | Human Anti-JAG1 Recombinant Antibody; Fab Fragment (HPAB-0944LY-F(E)) | ELISA | Human Fab |
| HPAB-0945LY-F(E) | Human Anti-JAG1 Recombinant Antibody; Fab Fragment (HPAB-0945LY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-617 | Human Anti-JAG1 Recombinant Antibody; scFv Fragment | WB, ELISA, FuncS | Human scFv |
| HPAB-0499-CN-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-0499-CN-S(P)) | ELISA, IF, Block | Human scFv |
| HPAB-0500-CN-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (HPAB-0500-CN-S(P)) | ELISA, IF, Block | Human scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0602CL | Mouse Anti-JAG1 Recombinant Antibody (TAB-0602CL) | FC, DB | Mouse IgG |
| TAB-0602CL-S(P) | Mouse Anti-JAG1 Recombinant Antibody; scFv Fragment (TAB-0602CL-S(P)) | ELISA | Mouse scFv |
| TAB-0603CL-S(P) | Mouse Anti-JAG1 Recombinant Antibody; scFv Fragment (TAB-0603CL-S(P)) | ELISA | Mouse scFv |
| TAB-0604CL-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (TAB-0604CL-S(P)) | ELISA | Human scFv |
| TAB-0605CL-S(P) | Human Anti-JAG1 Recombinant Antibody; scFv Fragment (TAB-0605CL-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0071CT | Recombinant Mouse anti-Human JAG1 Monoclonal antibody (EML134) | WB | |
| HPAB-0499-CN | Human Anti-JAG1 Recombinant Antibody (HPAB-0499-CN) | ELISA, IF, Block | Human IgG1 |
| HPAB-0500-CN | Human Anti-JAG1 Recombinant Antibody (HPAB-0500-CN) | ELISA, IF, Block | Human IgG1 |
| HPAB-1002LY | Human Anti-JAG1 Recombinant Antibody (HPAB-1002LY) | ELISA | Human IgG |
| HPAB-1003LY | Human Anti-JAG1 Recombinant Antibody (HPAB-1003LY) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-126-F (E) | Recombinant Human Anti-Jag1 Antibody Fab Fragment (JAG2.b33) | WB, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1607CQ | Recombinant Mouse Anti-JAG1 Antibody (CBL125) | FC, WB, Neut | IgG2 |
| NEUT-1608CQ | Recombinant Mouse Anti-JAG1 Antibody (4A24) | FC, Neut | IgG2 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1895 | Hi-Affi™ Recombinant Rabbit Anti-JAG1 Monoclonal Antibody (DS1895AB) | WB, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC326 | Recombinant Anti-JAG1 Vesicular Antibody, EV Displayed (VS-0425-YC326) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3683 | Anti-JAG1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3684 | Anti-Mouse JAG1 Immunohistochemistry Kit | IHC |
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